生物技术通报2026，Vol.42Issue(1)：241-250,10.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0482
蒺藜苜蓿bZIP转录因子MtbZIP29的克隆及功能分析
Cloning and Functional Analysis of a bZIP Transcription Factor MtbZIP29 from Medicago truncatula
摘要
Abstract
[Objective]Clone the MtbZIP29 gene from Medicago truncatula to study the self-activation,subcellular localization,and expression characteristics of the bZIP29 transcription factor,providing a theoretical research foundation for elucidating the involvement of the MtbZIP29 gene in the growth and development of M.truncatula and endogenous hormone signaling transduction.[Method]The MtbZIP29 gene was cloned from the wild-type M.truncatula'R108',and expression vectors were constructed for experimental purposes.Subcellular localization was observed using a transient fusion protein assay,and using yeast to analyze its self-activation activity.Bioinformatics analyses were conducted on the MtbZIP29 gene,including protein physicochemical property analysis,prediction of promoter cis-acting elements,and prediction of secondary and tertiary protein structures.Additionally,RT-qPCR was employed to analyze the expression patterns of MtbZIP29 across different tissues and under treatments with various hormones(ABA,SA,6-BA,IAA,and MeJA).Transgenic tobacco plants were obtained using Agrobacterium-mediated method,and the function of MtbZIP29 was analyzed.[Result]The MtbZIP29 gene was successfully cloned,with a coding sequence(CDS)length of 1,518 bp encoding 506 amino acids.The encoded protein had molecular weight of 55.830 kD,a theoretical isoelectric point(pI)of 6.82,an instability index of 63.60,and is classified as an unstable hydrophilic protein.Secondary structure prediction revealed that α-helixes accounted for 25.89%,extended strands for 0.59%,and the remaining 73.52%as random coils.Subcellular localization indicated the protein was localized to the nucleus.Yeast assays demonstrated the encoded protein has transcriptional self-activation activity.Expression profiling showed that MtbZIP29 transcripts were the most abundant in the leaves,significantly lower in the stems and pods compared to other tissues and markedly influenced by different hormone treatments.Analysis of MtbZIP29-overexpressing plants showed that the rhizomes of transgenic tobacco were significantly enlarged compared with wild-type tobacco.[Conclusion]The MtbZIP29 gene is involved in root growth and development,responding to different hormones,and may participate in the regulatory processes of root morphology construction and stress adaptability by integrating hormone signals.关键词
蒺藜苜蓿/MtbZIP29转录因子/表达特征/自激活/细胞核定位/烟草/农杆菌转化/启动子分析Key words
Medicago truncatula/MtbZIP transcription factor/expression pattern/self-activation/nuclear localization/tobacco/agrobacterium transformation/promoter analysis引用本文复制引用
张驰昊,刘晋囡,晁跃辉..蒺藜苜蓿bZIP转录因子MtbZIP29的克隆及功能分析[J].生物技术通报,2026,42(1):241-250,10.基金项目
内蒙古自治区科技厅内蒙古自治区科技重大专项项目(2022JBGS00160302) （2022JBGS00160302）
