生物技术通报2026，Vol.42Issue(1)：251-261,11.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0902
象草C2H2基因家族鉴定及表达分析
Identification and Expression Analysis of C2H2 Gene Family in Cenchrus purpureus
摘要
Abstract
[Objective]To identify C2H2-type zinc finger protein gene family members in Cenchrus purpureus(elephant grass)and explore their regulatory roles in cold stress responses,thereby laying a foundation for elucidating the functional mechanisms of C2H2 transcription factors.[Method]Based on the whole-genome data of elephant grass(Cenchrus purpureus),bioinformatics methods were used to systematically identify members of the C2H2 gene family.Transcriptome data and RT-qPCR were employed to analyze the expression patterns of these C2H2 genes under cold stress,and heterologous expression in Saccharomyces cerevisiae was performed to verify the functions of key genes.[Result]A total of 144 CpC2H2 genes were identified in C.purpureus.Phylogenetic analysis clustered these genes into 10 subgroups.Gene structure and conserved motif analyses revealed that members within the same subgroup exhibited highly conserved gene structures and conserved motifs.Cis-acting element analysis showed that 65 CpC2H2 genes contained cold-responsive cis-regulatory elements in their promoter regions.Expression pattern analysis indicated that the CpC2H2 gene family displayed distinct tissue-specific expression,among which CpC2H2-33,CpC2H2-107,CpC2H2-108,and CpC2H2-131 were significantly upregulated under cold stress.RT-qPCR results were consistent with transcriptome data.Heterologous expression experiments in S.cerevisiae confirmed that the encoded products of these four genes significantly enhanced the cold tolerance of recombinant strains.[Conclusion]A total of 144 C2H2 genes are identified in C.purpureus.The members of this family show distinct tissue specificity and differential expression under cold stress.Among them,CpC2H2-33,CpC2H2-107,CpC2H2-108,and CpC2H2-131 can enhance the cold tolerance of heterologous organisms,suggesting that they may play key roles in cold stress responses in C.purpureus.This study provides valuable insights for further investigating the functions of the C2H2 gene family and candidate genes for improving cold resistance in elephant grass through molecular breeding.关键词
象草/C2H2基因家族/低温胁迫/生物信息学Key words
Cenchrus purpureus/C2H2 gene family/cold stress/bioinformatics引用本文复制引用
杨丹,黄琳凯,严海东,靳雅荣,毛春力,王碧娴,张雅宁,杨智怡,周芷瑶,杨锐鸣,范恒睿..象草C2H2基因家族鉴定及表达分析[J].生物技术通报,2026,42(1):251-261,11.基金项目
国家重点研发计划(2024YFF1001300),四川省自然科学基金重点项目(2025ZNSFSC0019) （2024YFF1001300）
