天津中医药大学学报2026，Vol.45Issue(1)：45-54,10.DOI:10.11656/j.issn.1673-9043.2026.01.07
两地汤治疗绝经综合征机制研究
Mechanism study of Liangdi Decoction in treating menopausal syndrome
摘要
Abstract
[Objective]To explore the mechanism of Liangdi Decoction(LDD)in treating menopausal syndrome(MPS)based on network pharmacology and in vivo experiments.[Methods]The main active ingredients and target genes of LDD were obtained from databases including HERB(a high-throughput experiment-and reference-guided database of traditional Chinese medicine),PubChem,and SuperPred.Disease targets for MPS were retrieved from GeneCards,OMIM,and DrugBank.The common targets between LDD and MPS were selected and imported into the STRING database to construct a protein-protein interaction(PPI)network.The"drug-active ingredient-target"network was visualized using Cytoscape 3.10.3.Gene Ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using the DAVID database.Molecular docking of core active ingredients with key protein factors in predicted signaling pathways was conducted using AutoDock Tools 1.5.7 and CB-Dock2 to validate potential interactions.In vivo experiments were performed to measure the expression levels of interleukin-6(IL-6),signal transducer and activator of transcription 3(STAT3),phosphorylated STAT3(p-STAT3),Caspase-3,B-cell lymphoma-2(Bcl-2),and hypoxia-inducible factor-1α(HIF-1α),verifying the mechanism of LDD in ameliorating ovarian aging in MPS.[Results]Six core proteins(IL-6,STAT3,HIF-1α,Caspase-3,Bcl-2)and eight key active ingredients(coniferin,sugiol,catechin,isoimperatorin,marmesin,byakangelicol,acacetin,poncimarin)were screened.The molecular docking binding energies were all<-5 kcal/mol.Experiments showed that LDD could down-regulate the protein expression of IL-6,STAT3,HIF-1α,and caspase-3,and up-regulate the expression of Bcl-2.[Conclusion]LDD may delay ovarian aging by inhibiting the HIF-1 signaling pathway,regulating the expression of proteins such as IL-6,STAT3,Caspase-3,and Bcl-2,thereby reducing hypoxia-inducible factor levels and inflammation,and enhancing anti-apoptotic expression.关键词
两地汤/绝经综合征/炎症性老化/网络药理学/分子对接Key words
Liangdi Decoction/menopausal syndrome/inflammaging/network pharmacology/molecular docking分类
医药卫生引用本文复制引用
王若溪,鄢梁裕,杜小利..两地汤治疗绝经综合征机制研究[J].天津中医药大学学报,2026,45(1):45-54,10.基金项目
宁夏自然科学基金项目(2022AAC03216) （2022AAC03216）
宁夏区域高发病中西医结合防治研究重点实验室开放项目(4301240029) （4301240029）
宁夏回族自治区科技惠民专项项目(2023CMG03028) （2023CMG03028）
宁夏医科大学校级青年人才培育项目(BL202505000025). （BL202505000025）
