生物技术通报2026，Vol.42Issue(1)：338-351,14.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0600
桦褐孔菌醇通过激活Nrf2/PGC-1α/线粒体自噬防治AFB1诱导的小鼠肝损伤
Inotodiol Prevention of Aflatoxin B1-induced Liver Injury by Activating Nrf2/PGC-1α/Mitophagy
摘要
Abstract
[Objective]Inotodiol(INO),a triterpenoid compound extracted from the fungus Phaeoporus obliquus,a dual-purpose fungus for both medicine and food.It possesses biological activities such as antioxidant and anti-inflammatory properties.The protective effect of INO on the hepatotoxicity of aflatoxin B1(AFB1)was studied through in vivo and in vitro experiments.[Method]The mouse liver and Alpha mouse liver 12 cell(AML12)injury model were established using AFB1.Liver tissue sections were prepared to analyze the pathological changes.The antioxidant levels in liver tissues,serum and AML12 cells were analyzed through ELISA and antioxidant-related detection kits.Reactive oxygen species(ROS)and mitochondrial membrane potential(MMP)were detected by fluorescence microscopy.The expressions of Nrf2,PGC-1α,and mitophagy signaling pathway proteins in liver and AML12 cells were detected by Western blot and qPCR.[Result]AFB1 significantly increased the activities of CYP450,AST,and ALT in the liver and AML12 cells(P<0.01).Pretreatment with INO significantly reduced these proteins'expressions(P<0.01).INO significantly reversed(P<0.01)the decline in antioxidant capacity and mitochondrial dysfunction induced by AFB1.Analysis of Nrf2,PGC-1α and mitophagy signaling pathways revealed that INO significantly alleviated AFB1-induced suppression of these pathways,and ultimately attenuated oxidative damage and restoring mitochondrial dysfunction caused by AFB1.[Conclusion]INO inhibits AFB1-induced hepatotoxicity both in vitro and in vivo.The protective effect of INO on the liver is closely related to the activation of the Nrf2/PGC-1α signaling pathway and the promotion of mitophagy.关键词
桦褐孔菌醇/AFB1/肝损伤/氧化应激/线粒体自噬Key words
inotodiol/aflatoxin B1/liver injury/oxidative stress/mitophagy引用本文复制引用
康恺,杨微,李迎春,谢为天,吴海燕,尤育品,陈志宝..桦褐孔菌醇通过激活Nrf2/PGC-1α/线粒体自噬防治AFB1诱导的小鼠肝损伤[J].生物技术通报,2026,42(1):338-351,14.基金项目
广东省创新强校重点项目(2020ZDZX1043),广东海洋大学启动基金(R2006),广东海洋大学滨海农业学院杰出青年基金(BH2025JCQN007) （2020ZDZX1043）
