烟草科技2026，Vol.59Issue(2)：1-12,55,13.DOI:10.16135/j.issn1002-0861.2025.0562
GC-MS-MS结合GC-O分析卷烟主流烟气中的嗅觉活性硫化物
Analysis of odor-active sulfides in mainstream cigarette smoke by GC-MS-MS combined with GC-O
摘要
Abstract
To achieve accurate quantification and odor activity identification of sulfides in mainstream cigarette smoke,sulfides with odor activities reported in the research of food,environment,and agriculture were retrieved,and their union with those listed in the Rodgman&Perfetti list resulted in a target list of 65 sulfides.A quantitative method was established for 63 sulfides in the target list using GC-MS-MS combined with GC-MS,while semi-quantitative method was developed for carbonyl sulfide and hydrogen sulfide.A laboratory reference cigarette(Virginia type)and 1R6F reference cigarette(blended type)were analyzed.Based on the detection results,standard solutions were prepared to simulate the single-puff release of the smoke,and then the gas chromatography-olfactometry(GC-O)technology was used to analyze the odor characteristics of the sulfides in mainstream smoke,addressing the technical challenge of identifying odor characteristics of trace components in the complex smoke matrix.The results showed that 1)Among the 65 measured components,the intra-and inter-day RSDs for 62 target compounds were all no greater than 10%,indicating good method precision.The recovery rates of 61 target compounds ranged from 71%to 130%,indicating high method accuracy.The detection limits and quantification limits for 63 target compounds were 0.03‒45.88 ng/cig and 0.11‒131.95 ng/cig,respectively,indicating high sensitivity of the method.2)In the laboratory reference cigarette smoke,50 sulfides were identified,and 48 sulfides were detected in 1R6F reference cigarette smoke.Among these,20 sulfides were not reported in the smoke components of Rodgman&Perfetti list.3)Based on GC-O analysis,21 sulfides in mainstream cigarette smoke were identified to be odor active by using the odor intensity method and the flavor dilution(FD)method.Among which,nine compounds including methyl mercaptan,3-mercapto-2-pentanone,3-(methylthio)propionaldehyde,dimethyl trisulfide,2-acetyl-2-thiazoline,2-mercapto-3-pentanone,furfuryl mercaptan,2-methyl-3-mercaptofuran and thiophene-2-methanethiol made major contributions to the odor of cigarette smoke.关键词
卷烟/主流烟气/嗅觉活性/硫化物/气相色谱‒串联质谱(GC‒MS‒MS)/气相色谱‒嗅闻(GC‒O)Key words
Cigarette/Mainstream smoke/Odor activity/Sulfide/Gas chromatography-tandem mass spectrometry(GC-MS-MS)/Gas chromatography-olfactometry(GC-O)分类
轻工纺织引用本文复制引用
黄贝笛,黄延俊,王晓瑜,秦亚琼,李雨萱,张廷贵,邓其馨,张晓兵,李学杰,刘克建..GC-MS-MS结合GC-O分析卷烟主流烟气中的嗅觉活性硫化物[J].烟草科技,2026,59(2):1-12,55,13.基金项目
中国烟草总公司基础研究项目"基于风味组学和分子对接的烟气甜感关键成分组群研究"(110202403007). （110202403007）
