烟草科技2026，Vol.59Issue(2)：22-30,9.DOI:10.16135/j.issn1002-0861.2024.0651
感染黑胫病烟株根际土壤微生物群落结构和代谢物特征分析
Structure and metabolite characteristics of microbial community in rhizosphere soil of tobacco plants infected with black shank
摘要
Abstract
To clarify the structural and metabolic changes in microbial communities in the rhizosphere soil of healthy and black-shank-infected tobacco plants,soil samples from Nanzheng District,Hanzhong City,Shaanxi Province were analyzed by microbial metagenomics and metabolomics techniques.The results showed that:1)there was no significant difference in the diversity of microbial communities in the rhizosphere soil between healthy and infected tobacco plants,while the richness of microbial communities in the rhizosphere soil of infected tobacco plants was significantly higher than that of healthy plants.2)The relative abundance of genera such as Caulobacter,Dokdonella and Afipia in the rhizosphere soil of infected tobacco plants was significantly higher than that of healthy plants,while the relative abundance of genera such as Acinetobacter and Arthrobacter was extremely significantly or significantly lower.3)Analysis results of 213 significantly differential metabolites revealed that the relative abundance of sugars such as melibiose,stachyose,levan and α-lactose,and amino acids such as L-leucine,L-isoleucine and valine in the rhizosphere soil of infected tobacco plants was significantly higher than that of healthy plants,while the relative abundance of organic acids such as succinic acid,nonanoic acid,3-hydroxy-tetradecanedioic acid and salicylic acid was significantly lower.4)Correlation analysis results indicated that the relative abundance between metabolites such as stachyose,levan,valine and Acinetobacter,Arthrobacter in the rhizosphere soil of healthy and infected tobacco plants were highly significantly or significantly negatively correlated.Therefore,infection of tobacco plants with black shank altered the composition of metabolites in rhizosphere soil,indirectly affecting the microbial community structure and soil microecological balance.关键词
烟草/黑胫病/根际土壤/微生物/宏基因组/代谢物Key words
Tobacco/Black shank/Rhizosphere soil/Microorganism/Metagenomics/Metabolite分类
轻工纺织引用本文复制引用
李树泓,杜薇,张松杰,李先锋,杜鸿波,任天宝,张松涛,耿宗泽,杜娇..感染黑胫病烟株根际土壤微生物群落结构和代谢物特征分析[J].烟草科技,2026,59(2):22-30,9.基金项目
四川中烟工业有限责任公司科研项目"基于碳氮调节的宽窄·润甜香汉中烟叶提质增香技术研究"(yl202212) （yl202212）
河南农业大学2023年高层次人才专项支持基金项目(30501597). （30501597）
