新医学2026，Vol.57Issue(2)：139-148,10.DOI:10.12464/j.issn.0253-9802.2025-0349
MOV纳米粒的构建及其通过恢复气道免疫调节功能改善气道过敏性疾病的作用
Construction of MOV nanoparticles to improve allergic airway diseases by restoring airway immunomodulatory function
摘要
Abstract
Objective To construct a nanoparticle-based carrier capable of restoring airway immunoregulatory function under allergic conditions,and evaluate its immunomodulatory effects and potential therapeutic value against allergic airway inflammation.Methods Vasoactive intestinal peptide(VIP)-loaded nanoparticles(VIP NPs)were prepared using a double-emulsion method and further modified with an MHC-Ⅱ/OVA epitope peptide fusion protein to generate MOV nanoparticles(MOV NPs).The physicochemical properties and in vitro release profile of MOV NPs were characterized.An ovalbumin(OVA)-induced murine model of allergic airway disease was established and treated with different doses of MOV NPs.Serum allergen-specific antibodies,inflammatory mediators and Th2 cytokines in bronchoalveolar lavage fluid(BALF)were measured.Flow cytometry and functional assays were performed to analyze the abundance and suppressive activity of regulatory T cells(Tregs)in airway tissues.The expression levels of Treg-associated cytokines,transforming growth factor-β(TGF-β)and interleukin-10(IL-10)were further determined.Results MOV NPs exhibited a uniform spherical morphology,negative surface charge,stable sustained-release properties,and a VIP encapsulation efficiency of(63.12±2.15)%.MOV NPs treatment significantly reduced serum sIgG1 and sIgE levels,as well as BALF inflammatory mediators and Th2 type cytokines,in a dose-dependent manner.Compared with the model group,MOV NPs markedly increased the number and immunosuppressive function of airway Tregs,and up-regulated the expression levels of TGF-β and IL-10.Conclusion MOV NPs integrate dual functions of antigen presentation and VIP-mediated immunoregulation,which can effectively inhibit airway allergic inflammation and restore local immune homeostasis.关键词
MOV纳米粒/血管活性肠肽/调节性T细胞/免疫调控/气道过敏性疾病Key words
MOV nanoparticles/Vasoactive intestinal peptide/Regulatory T cells/Immune homeostasis/Allergic airway disease引用本文复制引用
莫丽华,胡宇琴,杨锦娜,罗向前..MOV纳米粒的构建及其通过恢复气道免疫调节功能改善气道过敏性疾病的作用[J].新医学,2026,57(2):139-148,10.基金项目
广东省基础与应用基础研究基金(2023A1515030299) （2023A1515030299）
深圳市科技计划资助项目(JCYJ20220530154013030,JCYJ20240813145213018) （JCYJ20220530154013030,JCYJ20240813145213018）
南方医科大学深圳医院院内基金腾飞计划项目(22H3ATF04) （22H3ATF04）
