新疆农业大学学报2025，Vol.48Issue(4)：287-295,9.DOI:10.20088/j.cnki.jxau.2025.04.004
耐盐菌的筛选及其对盐胁迫下黄瓜幼苗的缓解效应
Screening of Halotolerant Bacteria and Ameliorative Effects on Salt-Stressed Cucumber Seedlings
摘要
Abstract
To expand the resources of plant growth-promoting rhizobacteria capable of enhancing the salt toler-ance of crops.In this study,the cucumber variety'Jinyou 401'was used as the test material.From the rhizo-sphere soil of cucumber seedlings under 75 mmol/L NaCl stress,highly efficient pro-growth salt-tolerant bacteria were isolated and screened.Through pot inoculation test,when the seedlings grew to two leaves and one heart,four different treatments were set up,sterile water(CK),strain Rh72(Rh72),sterile water+75.0 mmol/L NaCl,strain Rh72+75.0 mmol/L NaCl,to explore the regulation effect of salt-tolerant bacteria Rh72 on the growth and salt tolerance of cucumber seedlings under salt stress.A total of 188 bacterial strains were isolated and purified from the rhizoidal soil of cucumbers treated with salt for 15 days.Through seed germination tests,11 bacterial strains with promoting growth and salt tolerance were screened out.Among them,bacterial Rh72 had the strongest promoting growth and salt tolerance for cucumber seeds.Phylogenetic trees were constructed by the adjacent connection method.The bacterial isolate Rh72 was identified as belonging to the genus Microbac-terium.The pot experiment demonstrated that compared with the untreated group,the inoculated group showed a 92.29%increase in plant height,a 63.61%increase in biomass,a significant 61.60%increase in root vitality and a 19.73%increase in chlorophyll a content(P<0.05),a significant 14.28%reduction in leaf electrolyte permeability(P<0.05),a significant 51.47%increase in superoxide dismutase(SOD)activity,a 21.00%in-crease in peroxidase(POD)activity and a 51.85%increase in catalase(CAT)activity(P<0.05),a signifi-cant 48.03%increase in proline content(P<0.05),and a significant 4.10%decrease in superoxide anion(O2·-),8.16%decrease in hydrogen peroxide(H2O2)and 28.39%decrease in malondialdehyde(MDA)con-tent(P<0.05).In conclusion,the salt-tolerant bacterium Rh72 can enhance the resistance of cucumber seed-lings to salt stress by increasing the biomass of cucumber seedlings,promoting the accumulation of osmotic sub-stances in the seedlings,enhancing the activity of antioxidant enzymes,and reducing the content of MDA.关键词
耐盐菌株/黄瓜幼苗/盐胁迫/抗氧化酶Key words
salt-tolerant strains/cucumber seedling/salt stress/antioxidant properties分类
农业科技引用本文复制引用
郭雨,牛婕,杨梦雪,李琬玉,葛俊豪,任紫琪,许红军,李红..耐盐菌的筛选及其对盐胁迫下黄瓜幼苗的缓解效应[J].新疆农业大学学报,2025,48(4):287-295,9.基金项目
国家自然科学基金(32360790) （32360790）
新疆维吾尔自治区重点研发资助项目(2022B02023-2) （2022B02023-2）
新疆维吾尔自治区蔬菜产业技术体系(XJARS-07) （XJARS-07）
