新疆农业大学学报2025，Vol.48Issue(4)：296-303,8.DOI:10.20088/j.cnki.jxau.2025.04.005
沙棘叶醇提物对鸡源大肠杆菌的体外抑菌活性和机制探究
In Vitro Antibacterial Effect of the Ethanol Extract from Sea Buckthorn Leaves Against Chicken-Originated Escherichia coli
摘要
Abstract
In order to explore the antibacterial activity,antibacterial mechanism and antibacterial stability of the ethanol extract of sea buckthorn leaves on chicken-originated Escherichia coli,the drug sensitivity test of 5 strains of E.coli from chicken was carried out by selecting 10 common antibiotics to determine their drug resis-tance characteristics.The ethanol extract was obtained by extracting the dry powder of sea buckthorn leaves with 75%ethanol.Five groups were set up:1.00,1.50,2.00 g/mL,positive control(polymyxin B)and blank con-trol(sterile normal saline).The Oxford cup method was used to determine the inhibition zone of the ethanol ex-tract of sea buckthorn leaves on the five isolated strains of E.coli from chickens.The minimum inhibitory con-centration(MIC)was determined by broth microdilution method.The contents of nucleic acid,protein,alkaline phosphatase(AKP)and lactate dehydrogenase(LDH)in the supernatant of bacterial liquid were detected by micro-enzyme labeling method.Coomassie brilliant blue staining was used to detect the change of soluble protein content in bacteria.The morphological changes of the bacteria were observed by scanning electron microscopy(SEM).The results showed that the five strains were resistant to lincomycin,tetracycline and vancomycin,par-tially resistant to amoxicillin,spectinomycin,ampicillin,erythromycin and streptomycin,and all sensitive to genta-micin and polymyxin B.The inhibition zone diameters of 1.00,1.50,2.00 g/mL ethanol extract of sea buck-thorn leaves on chicken Escherichia coli were(11.23±0.44)mm,(12.77±0.53)mm and(13.59±0.52)mm,respectively.The MIC of ethanol extract of seabuckthorn leaves against chicken E.coli was 125.00 mg/mL.Compared with the blank control,the contents of nucleic acid,protein and AKP in the supernatant of E.coli bacterial liquid increased significantly(P<0.05),the contents of LDH and soluble protein in the bacterial cells decreased significantly(P<0.05),and the surface of the bacteria showed depression,shrinkage and rupture.In summary,the ethanol extract of seabuckthorn leaves has good in vitro antibacterial activity against chicken-de-rived E.coli,and its potential mechanism is to destroy the integrity of cell wall,change the morphological struc-ture of bacteria,and inhibit LDH activity and soluble protein synthesis in bacteria.关键词
沙棘叶/提取物/鸡源大肠杆菌/体外抑菌/抑菌机制Key words
sea buckthorn leaves/extracts/chicken-derived Escherichia coli/in vitro antibacterial activity/antibacterial mechanism分类
农业科技引用本文复制引用
王利敏,韩俊成,马玉龙,何莹,朱琳,辛子惠,赵航,卫丁一,刘连荣,戴小华..沙棘叶醇提物对鸡源大肠杆菌的体外抑菌活性和机制探究[J].新疆农业大学学报,2025,48(4):296-303,8.基金项目
新疆维吾尔自治区"三区"人才支持计划科技人员专项计划项目(2025年度) （2025年度）
哈密市科学研究与技术开发计划项目(HM-2024-kj017) （HM-2024-kj017）
国家级大学生创业训练计划项目(202410758002X) （202410758002X）
国家级大学生创新训练计划项目(20241075801) （20241075801）
