针刺研究2026，Vol.51Issue(2)：189-198,10.DOI:10.13702/j.1000-0607.20250074
基于AMPK/ULK1信号通路研究温针灸调控慢性疲劳综合征大鼠线粒体自噬的作用机制
Effect of warming needle moxibustion on mitochondrial autophagy in rats with chronic fatigue syndrome based on AMPK/ULK1 signaling pathway
摘要
Abstract
Objective To observe the effect of warming needle moxibustion(WNM)on the expression of AMP-activated protein kinase(AMPK)/Unc-51 like autophagy activating kinase 1(ULK1)signaling pathway in chronic fatigue syndrome(CFS)rats,so as to explore its mechanism underlying improvement of CFS.Methods Male SD rats were randomly divided into control,model,WNM and coenzyme groups,with 10 rats in each group.The CFS model was established by multi-factor compound stress stimulation method(exhaustive swimming+chronic restraint+alternate-day fasting).The rats of the WNM group received warming needle moxibustion stimulation at bilateral"Zusanli"(ST36),"Guanyuan"(CV4)and"Zhongwan"(CV12)for 15 min,once daily for 14 days.The rats of the coenzyme group were administered coenzyme Q10(1 mg/kg)by gavage once daily for 14 days.Body weight and swimming time of the rats were recorded before and after modeling and after intervention.Behavioral changes were assessed using the open-field test.Histological changes in skeletal muscle were observed via HE staining.The structure of mitochondria and autophagosomes in skeletal muscle were observed using transmission electron microscopy.The protein expression levels of AMPK,phosphorylated(p)-AMPK,ULK1,p-ULK1,microtubule-associated protein 1 light chain 3(LC3)-Ⅰ and LC3-Ⅱ in skeletal muscle were detected by Western blot,and the ratios of p-AMPK/AMPK,p-ULK1/ULK1 and LC3-Ⅱ/LC3-Ⅰ were calculated.The mRNA expression levels of AMPK,ULK1 and LC3 in skeletal muscle were detected by real-time PCR.Results Compared with the control group,the body weight,swimming time,and the duration of standing and the number of grid crossing were significantly reduced(P<0.05),while the mRNA expression levels of ULK1 and LC3,and the ratio of p-ULK1/ULK1 were significantly increased in the model group(P<0.05).Compared with the model group,the body weight,swimming time,and the duration of standing and the number of grid crossing were significantly increased(P<0.05),and the mRNA expression levels of AMPK,ULK1 and LC3,as well as the ratios of p-ULK1/ULK1 and LC3-Ⅱ/LC3-Ⅰ,were significantly up-regulated in both the WNM and coenzyme groups(P<0.05).Conclusion Warming needle moxibustion can alleviate chronic fatigue syndrome by activating the AMPK/ULK1 pathway,upregulating the expression of AMPK/ULK1/LC3,and promoting mitochondrial autophagy,thereby enhancing mitochondrial morphology.关键词
慢性疲劳综合征/腺苷酸活化蛋白激酶/Unc-51样激酶信号通路/温针灸/线粒体自噬/骨骼肌Key words
Chronic fatigue syndrome/AMPK/ULK1 signaling pathway/Warming needle moxibustion/Mitochondrial autophagy/Skeletal muscle引用本文复制引用
原艺娜,刘娣,刘君伟,李花园,李龙,武永利..基于AMPK/ULK1信号通路研究温针灸调控慢性疲劳综合征大鼠线粒体自噬的作用机制[J].针刺研究,2026,51(2):189-198,10.基金项目
宁夏自然科学基金项目(No.2022AAC03532) （No.2022AAC03532）
