云南民族大学学报（自然科学版）2026，Vol.35Issue(1)：1-7,7.DOI:10.3969/j.issn.1672-8513.2026.01.001
扭肚藤多糖提取工艺优化及体外抗炎活性评价
Optimization of extraction process and anti-inflammatory activity in vitro evaluation of polysaccharides from Jasminum elongatum
摘要
Abstract
To optimize the ultrasonic extraction process of polysaccharides from Jasminum elongatum(Bergius)Willd.and study its anti-inflammatory activity in vitro.The total polysaccharide yield from Jasminum elongatum served as the primary response indicator.Ultrasound power,time,extraction temperature,and liquid-solid ratio were selected as independent variables to optimize the extraction process using both single-factor experiments and response surface methodology(RSM).Additionally,the impact on inflammatory factors released by LPS-stimulated RAW264.7 cells was evaluated.The optimal ultrasonic extraction parameters for Jasminum elongatum polysaccharides was obtained as follows:ultrasonic time of 33 min,ultrasonic power of 341 W,extraction temperature of 62 ℃,liquid-solid ratio of 39 mL/g.Under these conditions,the polysaccharide yield reached(6.98±0.34)％.The polysaccharides from Jasminum elongatum exhibited a dose-dependent inhibited the LPS-induced production of TNF-a,IL-1β,and IL-6 while enhancing the generation of IL-10.The optimized ultrasonic extraction process of polysaccharides from Jasminum elongatum is stable and feasible.The polysaccharides from Jasminum elongatum demonstrated anti-inflammatory activity in vitro,which provides theoretical basis for the further research on Jasminum elongatum.关键词
扭肚藤/多糖/超声提取工艺/抗炎活性Key words
Jasminum elongatum(Bergius)Willd./polysaccharides/ultrasonic extraction process/anti-inflammatory activity分类
医药卫生引用本文复制引用
李娟,方佳慧,何芳..扭肚藤多糖提取工艺优化及体外抗炎活性评价[J].云南民族大学学报（自然科学版）,2026,35(1):1-7,7.基金项目
十堰市太和医院科研项目(2023JJXM092) （2023JJXM092）
湖北省教育厅项目(2014CFB314). （2014CFB314）
