局解手术学杂志2026，Vol.35Issue(2)：139-144,6.DOI:10.11659/jjssx.12E024109
和厚朴酚调节mTOR/HIF-1α/VEGF信号通路对烟曲霉菌性角膜炎大鼠角膜组织损伤的影响
Impact of honokiol on corneal tissue damage in rats with Aspergillus fumigatus keratitis by regulating mTOR/HIF-1α/VEGF signaling pathway
摘要
Abstract
Objective To explore the impact of honokiol(HNK)on corneal tissue damage in rats with aspergillus fumigatus keratitis(AFK)by regulating mammalian target of rapamycin(mTOR)/hypoxia inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods SD rats were randomly divided into the Ctrl group(5 µL DMSO solvent),model group(5 µL DMSO solvent),low-dose HNK(L-HNK)group(2 µg/mL HNK),medium-dose HNK(M-HNK)group(4 µg/mL HNK),high-dose HNK(H-HNK)group(8 µg/mL HNK),and mTOR activator+H-HNK group(10 mg/kg MHY1485+8 µg/mL HNK).Except for the Ctrl group,the AFK rat models were established by the corneal contact lens method in the other groups.Slit-lamp microscopy was used to observe and evaluate the degree of corneal tissue damage.Plate colony counting was employed to assess aspergillus fumigatus content in corneal tissues.HE staining was performed to examine the histopathological changes in corneal tissues.ELISA was utilized to detect the levels of inflammatory cytokines interleukin(IL)-1β,IL-6,and tumor necrosis factor alpha(TNF-α)in corneal tissues.And Western blot was conducted to determine the protein expression levels of mTOR,HIF-1α,and VEGF in corneal tissues.Results Compared with the Ctrl group,the model group showed increased corneal tissue injury scores,colony counts,and elevated expression of IL-1β,TNF-α,and IL-6(P<0.05),along with disordered collagen fiber arrangement,swelling,extensive inflammatory cell infiltration,and necrotic tissue shedding.In contrast to the model group,the L-HNK group,M-HNK group and H-HNK group exhibited reduced corneal tissue injury scores,colony counts,and decreased expression of IL-1β,TNF-α,and IL-6(P<0.05),along with alleviated corneal tissue changes,the most pronounced in the H-HNK group.Compared with the H-HNK group,the mTOR activator+H-HNK group showed increased corneal tissue injury scores,colony counts,and elevated expression of IL-1β,TNF-α,and IL-6(P<0.05),along with aggravated corneal tissue changes.Compared with the Ctrl group,the model group demonstrated increased protein expression of mTOR,HIF-1α,and VEGF in the corneal tissue(P<0.05).Compared with the model group,the H-HNK group exhibited decreased expression of these proteins(P<0.05).Compared with the H-HNK group,the mTOR activator+H-HNK group showed increased expression of these proteins(P<0.05).Conclusion HNK can alleviate corneal tissue damage in AFK rats,which may be achieved by inhibiting the mTOR/HIF-1α/VEGF signaling pathway.关键词
和厚朴酚/mTOR/HIF-1α/VEGF信号通路/烟曲霉菌性角膜炎/角膜组织损伤Key words
honokiol/mTOR/HIF-1α/VEGF signaling pathway/aspergillus fumigatus keratitis/corneal tissue damage分类
医药卫生引用本文复制引用
王敏,霍楠,安伟乔,赵军波..和厚朴酚调节mTOR/HIF-1α/VEGF信号通路对烟曲霉菌性角膜炎大鼠角膜组织损伤的影响[J].局解手术学杂志,2026,35(2):139-144,6.基金项目
邯郸市科学技术研究与发展计划项目(21422083122) （21422083122）
