中国比较医学杂志2026，Vol.36Issue(1)：1-11,11.DOI:10.3969/j.issn.1671-7856.2026.01.001
补肾温阳化瘀方调节Notch1/Twist1介导的上皮-间质转化治疗子宫内膜异位症的机制研究
Mechanism of Bushen Wenyang Huayu formula in regulating Notch1/Twist1 mediated epithelial-mesenchymal transition in the treatment of endometriosis
摘要
Abstract
Objective To explore the mechanism of Bushen Wenyang Huayu formula in regulating the Notch1/Twist1 signaling pathway-mediated epithelial-mesenchymal transition(EMT)in the treatment of endometriosis.Methods SD female rats were randomly divided into Sham operation group,Model group,BWH-H group,BWH-L group,and Dienogest group.The EMs rat model of Kidney Yang Deficiency was constructed by autologous transplantation combined with ice water bath.After the modeling was completed,BWH-H group and BWH-L group were respectively given high and low-dose medicinal solutions of Bushen Wenyang Huayu formula by gavage.Dienoges group was given dienogest tablets by gavage,while Sham operation group and Model group were given an equal volume of distilled water by gavage for 21 consecutive days,weight,volume,and adhesion degree of ectopic lesions and uteri were observed.Serum levels of sex hormones were determined by enzyme-linked immunosorbent assay,E-Cadherin,N-Cadherin,Vimentin,Notch1,and Twist1 proteins were detected by immunohistochemistry and Western blot,and gene expression levels of E-Cadherin,N-Cadherin,Vimentin,Notch1,and Twist1 were detected by RT-qPCR.Results Serum levels of estradiol(E2),progesterone(P),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)were significantly increased in Model group compared with Sham operation group(P<0.05).N-Cadherin,Vimentin,Notch1,and Twist1 protein and mRNA levels in uterine tissue were also significantly increased(P<0.01,P<0.05),while E-Cadherin protein and mRNA levels were significantly decreased(P<0.01).The volume and weigh of ectopic lesions was significantly reduced in BWH-H group and BWH-L group traditional Chinese medicine and Dienogest group,compared with Model group(P<0.05,P<0.01),while E2,P,FSH,and LH levels were significantly decreased(P<0.05),N-Cadherin,Vimentin,Notch1,and Twist1 protein and mRNA levels in the uterine and ectopic lesion tissues were significantly decreased(P<0.01,P<0.05),and E-Cadherin protein and mRNA levels were significantly increased(P<0.01).Conclusions Bushen Wenyang Huayu formula can significantly inhibit the progression of endometriosis,and its mechanism may be related to the down-regulation of the Notch1/Twist1 signaling pathway,thereby regulating the EMT process.关键词
补肾温阳化瘀方/子宫内膜异位症/上皮-间质转化/Notch1/Twist1 信号通路Key words
Bushen Wenyang Huayu formula/endometriosis/epithelial-mesenchymal transition/Notch1/Twist1 signaling pathway分类
医药卫生引用本文复制引用
齐嘉泽,杨新宇,张丽美,孟鑫,马艺鸣,刘慧敏,陈景伟..补肾温阳化瘀方调节Notch1/Twist1介导的上皮-间质转化治疗子宫内膜异位症的机制研究[J].中国比较医学杂志,2026,36(1):1-11,11.基金项目
河北省自然科学基金资助项目(H2024423097,H2025423077) （H2024423097,H2025423077）
国家自然科学基金项目(82474558). （82474558）
