中国瓜菜2026，Vol.39Issue(2)：60-71,12.DOI:10.16861/j.cnki.zggc.2025.0416
基于广泛靶向代谢组学分析鸟巢蕨栽培嫩叶与老叶次生代谢特征
Comparative study on secondary metabolites characteristics of cultivat-ed tender and mature leaves of Asplenium nidus based on widely targeted metabolomics
摘要
Abstract
Using Asplenium nidus cultivated in Pu'er city's modern agricultural base as experimental material,a widely tar-geted metabolomics analysis was performed on mature leaves(C group)and tender leaves(D group)via ultra-high-perfor-mance liquid chromatography quadrupole time-of-flight tandem mass spectrometry(UHPLC-QTOF-MS/MS).Orthogo-nal partial least squares discriminant analysis(OPLS-DA)was employed to screen differential metabolites,and Kyoto En-cyclopedia of Genes and Genomes(KEGG)pathway enrichment was conducted to reveal metabolic regulatory networks,thereby elucidating the molecular mechanisms underlying secondary metabolic differences between tender and mature leaves during artificial cultivation.The results showed that a total of 647 metabolites were identified in the leaves of Asple-nium nidus,with 276 significantly differential metabolites identified,including 198 up-regulated and 78 down-regulated.KEGG pathway enrichment analysis revealed that the biosynthesis of alkaloids such as tropane derivatives,as well as fla-vonoid and flavonol biosynthesis pathways,played crucial roles in the observed metabolic differences.In conclusion,leaf age significantly influences the metabolomic profile of Asplenium nidus.Steroidal alkaloids are markedly up-regulated in tender leaves,whereas flavonoids are enriched in mature leaves,with differential metabolites mainly concentrated in alka-loid and flavonoid biosynthetic pathways.These results reveal the metabolic regulation characteristics of A.nidus at differ-ent leaf ages and provide a scientific basis for optimizing harvest time,developing bioactive compounds,and evaluating-medicinal value.关键词
鸟巢蕨/甾体生物碱/黄酮类/代谢异质性Key words
Asplenium nidus/Steroidal alkaloids/Flavonoids/Metabolic heterogeneity分类
农业科技引用本文复制引用
赵王瑞,周春燕,罗丹,董诗浪,徐振淮,王步天,林蓉,曲鹏,葛宇,杜华波..基于广泛靶向代谢组学分析鸟巢蕨栽培嫩叶与老叶次生代谢特征[J].中国瓜菜,2026,39(2):60-71,12.基金项目
国家天然橡胶产业技术体系普洱综合试验站(CARS-33-YN4) （CARS-33-YN4）
云南省黄华孙专家工作站(202305AF150125) （202305AF150125）
孟连县农民院士科技服务站 （）
云南农业大学本科教学改革工程项目(YNAUJG2024010) （YNAUJG2024010）
云南农业大学热带作物学院教育教学改革研究项目(202405) （202405）
