中国药科大学学报2026，Vol.57Issue(1)：1-10,10.DOI:10.11665/j.issn.1000-5048.2025041601
表面蛋白表征方法及其在疾病靶标发现中的应用
Surface protein characterization methods and their applications in disease target discovery
摘要
Abstract
Surface proteins play pivotal roles in physiological processes,including cell recognition,signal transduction,substance transport,and immune responses.However,challenges persist in characterizing abnormal surface proteins in disease states and identifying therapeutic targets,due to the low abundance of these proteins within the total proteome and the frequent presence of their complex glycosylation modifications.Recent years have witnessed the vigorous development of chemical proteomics,leading to the successful creation of various chemical probes for the labeling and characterization of cell surface proteins.These techniques have subsequently been applied to the detection of disease surface proteins and the discovery of corresponding targets.Surface protein characterization techniques based on chemical proteomics are discussed herein,focusing on the principles of amino acid-targeted labeling,proximity labeling,and glycoprotein capture.The novelty,advantages,and limitations of techniques such as targeted lysine labeling,peroxidase and photocatalytic proximity labeling,and chemical glycan capture and metabolic glycan labeling are elaborated,and their applications across various biological models and disease types are described,aiming to provide some reference for target discovery and drug d evelopment targeting surface proteins.关键词
表面蛋白/化学探针/肿瘤免疫/靶标发现Key words
surface proteins/chemical probe/tumor immunology/target discovery分类
医药卫生引用本文复制引用
张越阳,邵畅,郝海平,叶慧..表面蛋白表征方法及其在疾病靶标发现中的应用[J].中国药科大学学报,2026,57(1):1-10,10.基金项目
国家自然科学基金项目(No.82322068,No.82404703) （No.82322068,No.82404703）
江苏省自然科学基金项目(BK20220088,BK20241590) This study was supported by the National Natural Science Foundation of China(No.82322068,No.82404703) （BK20220088,BK20241590）
and the Natural Science Foundation of Jiangsu Province(BK20220088,BK20241590) （BK20220088,BK20241590）
