中国药理学通报2026，Vol.42Issue(2)：282-290,9.DOI:10.12360/CPB202505078
基于非靶向代谢组学探讨抗生素加重小鼠变应性鼻炎机制
Exploring the mechanism of antibiotic-exacerbated allergic rhinitis in mice based on untargeted metabolomics
摘要
Abstract
Aim To investigate the mechanism under-lying the exacerbation of allergic rhinitis(AR)in mice following antibiotic-induced gut microbiota depletion.Methods Ovalbumin(OVA)-induced AR mouse models and antibiotic cocktail-induced gut microbiota-depleted AR models were established.The frequency of nose-scratching and sneezing was recorded.Plasma OVA-specific immunoglobulin E(OVA-sIgE)levels and interleukin(IL)-6,IL-13,and IL-17 levels in bronchoalveolar lavage fluid(BALF)were measured using enzyme-linked immunosorbent assay(ELISA).Nasal mucosal pathology was evaluated via hematoxylin-eosin(HE)staining.Differential plasma metabolites were identified using untargeted metabolo-mics,and the associated pathways were predicted.Re-sults After gut microbiota depletion,intestinal mi-crobial diversity significantly decreased.Mice exhib-ited increased nose-scratching and sneezing frequen-cies,elevated plasma OVA-sIgE,and upregulated IL-6,IL-13,and IL-17 levels in BALF.Nasal mucosa displayed inflammatory lesions and goblet cell hyper-plasia.A total of 1 175 compounds were matched through a database of compounds,with 11 signifi-cantly altered metabolites:phenylpropanoids,polyketides,organic acids/derivatives,and lipids/lipid-like molecules were upregulated,while benzene-type compounds and organic oxygen compounds were downregulated.KEGG enrichment analysis revealed upregulated pathways including bile secretion,taste transduction,and glycerophospholipid metabolism.Conclusions Gut microbiota depletion exacerbates AR symptoms in mice,potentially by modulating me-tabolites(phenylpropanoids,polyketides,organic acids,lipids,benzene-type compounds)and pathways related to bile secretion,taste transduction,and glyc-erophospholipid metabolism.关键词
变应性鼻炎/抗生素/肠道菌群/免疫平衡/宏基因组学/非靶向代谢组学Key words
allergic rhinitis/antibiotics/gut micro-biota/immune balance/metagenomics/untargeted metagenomics分类
医药卫生引用本文复制引用
马健华,黄丽萍,徐贝,刘波,章常华,张雪平,柴琪,王帅康,王晶晶,段飞鹏..基于非靶向代谢组学探讨抗生素加重小鼠变应性鼻炎机制[J].中国药理学通报,2026,42(2):282-290,9.基金项目
国家自然科学青年基金资助项目(No 82205205) （No 82205205）
江西中医药大学校级科技创新团队发展计划(No CXTD220007) （No CXTD220007）
中药药理江西省重点实验室(No 2024SSY07111) （No 2024SSY07111）
