中国农学通报2026，Vol.42Issue(2)：191-197,7.
同心红葱软腐病株与健株内生细菌多样性及群落组成分析
Analysis of Endophytic Bacteria Diversity and Community Composition in Soft Rot and Healthy Plant of Allium fistulosum in Tongxin of Ningxia
摘要
Abstract
Allium fistulosum is one of the main characteristic economic crops in Tongxin,Ningxia,but the occurrence of soft rot disease affected the yield and quality of A.fistulosum.The analysis of the composition and diversity of endophytic bacterial communities related to the soft rot of A.fistulosum aims to provide a theoretical basis for understanding the mechanism of soft rot disease occurrence and determining the pathogenic bacteria.16S rDNA high-throughput sequencing technology and traditional isolation methods were used to compare the commonalities and differences of endophytic bacterial communities in healthy and soft rot plants of A.fistulosum.A total of 1334 OTUs were obtained from healthy and diseased samples,and the richness and diversity of endophytic bacteria were lower in diseased samples than in healthy samples.There were significant differences in endophytic bacterial communities between the two samples,with Proteobacteria as the dominant phylum,and its relative abundance in diseased and healthy plants was 82.51％and 43.49％,respectively.At the genus level,unclassified Enterobacteriaceae was the dominant genus in diseased samples(46.59％),while Chryseobacterium(11.98％),Pseudomonas(7.10％),and Flavobacterium(5.01％)were dominant genera in healthy samples.The bacterial strains isolated from healthy and diseased samples belonged to 11 genera,including 4 genera endemic to healthy plants,2 genera endemic to diseased plants,and 5 genera owned by both.Among them,Bacillus and Microbacterium were endemic genera to healthy plants with high isolation frequency,which may be related to disease resistance of healthy A.fistulosum;Klebsiella and Pseudomonas were common genera,and had a high isolation frequency in diseased plants.This study confirmed that the changes of endophytic bacterial diversity and community composition in A.fistulosum were closely related to the occurrence of soft rot disease,but the identification of pathogenic bacteria still requires screening and detection using multiple methods.关键词
红葱/软腐病/内生细菌/16S rDNA/高通量测序Key words
Allium fistulosum/soft rot disease/endophytic bacteria/16S rDNA/high-throughput sequencing分类
农业科技引用本文复制引用
马娟,喇真,胡明珠,代金霞..同心红葱软腐病株与健株内生细菌多样性及群落组成分析[J].中国农学通报,2026,42(2):191-197,7.基金项目
宁夏重点研发计划项目"退砂地土壤质量提升与复合栽培技术研究与示范"(2022BBF02025) （2022BBF02025）
宁夏大学创新项目"健康与软腐病红葱内生细菌多样性及群落结构差异"(202410749402). （202410749402）
