中国药理学通报2026，Vol.42Issue(2)：291-296,6.DOI:10.12360/CPB202505038
白藜芦醇通过抑制TGF-β信号通路抑制膀胱癌5637细胞迁移、侵袭和上皮间质转化
Resveratrol inhibits migration,invasion and EMT of bladder cancer 5637 cells through suppression of TGF-β signaling pathway
摘要
Abstract
Aim To study the effects of resveratrol(RSV)on migration and invasion of bladder cancer 5637 cells as well as the possible mechanisms.Meth-ods Sulforhodamine B(SRB)assay was used to de-tect the proliferation of 5637 cells.Transwell method was used to examine the migration and invasion of 5637 cells.Gelatin zymography was employed to de-tect the matrix metalloproteinase-2/-9(MMP-2/-9)se-cretion by 5637 cells.Western blot was applied to de-termine the expressions of marker proteins of EMT and associated signal molecules such as Smad2/3,p-38 MAPK,Akt,ERK.Results Treatment with RSV for 24,48 and 72 h inhibited the proliferation of 5637 cells significantly.RSV at the concentrations of 13.75,27.5,55,110 μmol·L-1 inhibited the migra-tion,invasion and MMP-2 secretion of 5637 cells.Western blot analysis indicated that RSV could reverse the transforming growth factor-β(TGF-β)-induced EMT and the phosphorylation of Smad2/3,p-38 MAPK and Akt.Conclusion RSV inhibits the migra-tion,invasion,secretion of MMP-2 and TGF-β-in-duced EMT of bladder cancer 5637 cells through sup-pression of TGF-β signaling pathway.关键词
白藜芦醇/膀胱癌/侵袭/迁移/上皮-间质转化/TGF-βKey words
resveratrol/bladder cancer/invasion/migration/EMT/TGF-β分类
医药卫生引用本文复制引用
黄双盛,高静,李彩丽,辛英,李燕..白藜芦醇通过抑制TGF-β信号通路抑制膀胱癌5637细胞迁移、侵袭和上皮间质转化[J].中国药理学通报,2026,42(2):291-296,6.基金项目
西北民族大学中央高校基本科研业务费资金资助项目(No 31920180135) （No 31920180135）
国家自然科学基金地区基金项目(No 81260330) （No 81260330）
