中国药科大学学报2026，Vol.57Issue(1)：46-53,8.DOI:10.11665/j.issn.1000-5048.2025041402
基于多成分定量联合化学计量学的不同产地通关藤质量评价
Quality evaluation of Marsdenia tenacissimae from different producing areas based on multi-component quantitative combined with chemometrics
摘要
Abstract
A quantitative method for the analysis of the multi-component contents in Marsdenia tenacissimae was established,and the quality differences were evaluated by principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA),factor analysis(FA)and weighted technique for order preference by similarity to ideal solution(TOPSIS)method.The contents of chlorogenic acid,cryptochlorogenic acid,sinapic acid,tenacigenoside A,tenacissoside G,tenacissoside I,tenacissoside H,drevogenin A,betulinic acid and lupeol were determined by HPLC wavelength switching method.At the same time,the contents of alcohol-soluble extract and total ash were detected.PCA,OPLS-DA and FA methods were used to identify the origin of M.tenacissimae from different producing areas.According to the OPLS-DA model,the index weight was determined to construct the weighted TOPSIS evaluation model.The qualities of M.tenacissimae from different producing areas were analyzed by model scoring results.The contents of 12 indexes in 18 batches of M.tenacissimae varied to different degrees,and the repeatability and accuracy of the test method were satisfactory.P CA analysis divided 18 batches of M.tenacissimae into three categories.OPLS-DA identified five main potential quality markers,including tenacissoside A,tenacissoside I,lupeol,tenacissoside H and chlorogenic acid.The evaluation results of FA and weighted TOPSIS method were consistent,which showed that the quality of M.tenacissimae from Yunnan and Guizhou was better.The established multi-component quantitative analysis method is accurate and reliable,the chemometrics model has strong predictive ability,and the evaluation results of FA and weighted TOPSIS method are scientific and objective.The combination of the four methods can clearly determine the qualities of M.tenacissimae from different producing areas.关键词
通关藤/高效液相色谱法/化学计量学/加权TOPSIS法/综合评价Key words
Marsdenia tenacissimae/HPLC/chemometrics/weighted TOPSIS/comprehensive evaluation分类
医药卫生引用本文复制引用
龙悦,胡阳,贺玲,祝丽超,邵莉..基于多成分定量联合化学计量学的不同产地通关藤质量评价[J].中国药科大学学报,2026,57(1):46-53,8.基金项目
辽宁省中药传承技术骨干人才培养项目(辽中医药综合字[2021]70号) （辽中医药综合字[2021]70号）
辽宁省中医药特色人才培养项目(辽中医药综合字[2021]38号) （辽中医药综合字[2021]38号）
辽宁省教育厅高等学校基本科研项目(LJKZ0893) This study was supported by the Training Projects for Traditional Chinese Medicine Inheritance Technical Backbone Talent of Liaoning Province(LZYYZH[2021]No.70),the Training projects for Chinese Medicine Characteristic Talent of Liaoning Province(LZYYZH[2021]No.38)and the Basic Scientific Research Projects of Colleges and Universities of Liaoning Provincial Department of Education(LJKZ0893) （LJKZ0893）
