中国药理学通报2026，Vol.42Issue(2)：322-331,10.DOI:10.12360/CPB202506038
高尔基体形态变化与A1型星形胶质细胞关联
Association between Golgi apparatus morphological alteration and A1 astrocytes
摘要
Abstract
Aim To investigate the relationship be-tween Golgi morphology and astrocyte activation.Methods CTX-TNA2 cells were divided into the fol-lowing groups:the control group,the astrocyte condi-tioned medium(ACM)group,the ACM+10 µmol·L-1 calycosin(CAL)group,the ACM+25 µmol·L-1 CAL group,and the ACM+50 µmol·L-1 CAL group.ACM,a pre-prepared culture medium,was used for inducing A1 astrocyte.In the ACM group,cells were stimulated with ACM for 24 h.In the CAL+ACM groups,cells were pretreated with the corresponding concentration of CAL for 6 hours before being stimu-lated with ACM for an additional 24 h.First,the cells in the control group and the ACM group were incu-bated with DMEM high-glucose medium containing Golgi apparatus fluorescent probes.The morphology of the Golgi apparatus was subsequently observed using the MICA full-scene microscopic imaging analysis plat-form.Next,real-time fluorescence quantitative PCR and cellular immunofluorescence were employed to measure the indicators related to A1 astrocyte activa-tion to verify the regulatory effect of CAL on A1 astro-cyte activation.Third,after ACM-stimulated AS were treated with CAL,the gradual changes in Golgi appara-tus morphology upon inhibition of astrocyte activation were observed by MICA full-scene microscopic imag-ing analysis platform.Meanwhile,using Western blot or immunofluorescence,we further examined the ex-pression alterations of proteins associated with Golgi apparatus morphology in ACM-stimulated astrocytes after treatment with CAL,and the changes in the sig-naling pathways associated with Golgi fragmentation.Results The observation of Golgi apparatus morphol-ogy revealed that,compared with the control group,the ACM group exhibited dissociation of the Golgi structure and the formation of a large number of dis-persed vesicles.PCR and immunofluorescence analy-ses demonstrated that CAL intervention dose-dependently attenuated the ACM-induced C3,iNOS,and IL-1β mRNA expressions and the activation of sig-nal molecules related to A1 astrocyte activation.Fur-ther fluorescence probe staining of the Golgi apparatus suggested that,CAL treatment of A1 astrocytes could dose-dependently diminish the formation of Golgi appa-ratus vesicles within astrocytes.Additionally,West-ern blot and immunofluorescence results indicated that CAL could dose-dependently prevent the decrease in the expression of proteins associated with Golgi appara-tus morphology induced by ACM,and block the activa-tion of the UNC-5-like kinase 1/Ras-like GTPase 9 signaling pathway.Conclusions The fragmentation of the Golgi apparatus exhibits a synchronous correla-tion with the A1 astrocyte activation and may play a role in the evolutionary development of A1 astrocytes.关键词
星形胶质细胞/A1/高尔基体/碎片化/毛蕊异黄酮/NLRP3炎症小体Key words
astrocytes/A1/Golgi apparatus/frag-mentation/CAL/NLRP3 inflammasome分类
医药卫生引用本文复制引用
周龙云,曾南海,方露,姚敏,刘书芬..高尔基体形态变化与A1型星形胶质细胞关联[J].中国药理学通报,2026,42(2):322-331,10.基金项目
国家自然科学基金青年科学基金资助项目(No 82205148) （No 82205148）
国家自然科学基金面上基金资助项目(No 82374476) （No 82374476）
