中国药科大学学报2026，Vol.57Issue(1)：54-59,6.DOI:10.11665/j.issn.1000-5048.2025032501
LC-MS/MS法测定小型猪血浆中泰妙菌素浓度及其在不同制剂药代动力学研究中的应用
Determination of tiamulin concentration in minipig plasma by LC-MS/MS and its application in pharmacokinetic studies of different formulations
涂佳卉 1耿梅 2侯庆明 2罗贤海 1邱志霞 1王聪3
作者信息
- 1. 中国药科大学创新药物药代动力学评价平台,南京 210009
- 2. 山东胜利生物工程有限公司,济宁 272000
- 3. 中牧实业股份有限公司,北京 100070
- 折叠
摘要
Abstract
The present study aimed to establish an LC-MS/MS method for the quantification of tiamulin in minipig plasma and to further conduct a pharmacokinetic comparison of different formulations.The plasma samples were extracted with acetonitrile(meloxicam as internal standard),separated on a C18 column,and quantified by multiple reaction monitoring mode(ESI+).Sanyuan minipigs were used as experimental animals.Plasma samples were collected after intravenous injection(10 mg/kg)and intragastric administration(20 mg/kg).The method showed good linearity,with intra-and inter-batch RSD of 1.00%-8.13%and RE within±15%.The extraction recovery,matrix effect and stability of the analytical methods met the relevant requirements.Tiamulin fumarate active pharmaceutical ingredient was intravenously administered,with c0 of about(4 383.73±2 676.78)ng/mL,AUC0-t of about(4 803.50±965.68)h·ng/mL,t1/2 of about(4.66±1.68)h,and CL of about(2.14±0.46)L/(kg·h).Three tiamulin formulations were intragastrically administered,with cmax of(552.00±328.55),(545.00±136.97)and(590.60±237.02)ng/mL,tmax of(1.47±0.68),(0.69±0.75)and(0.72±0.72)h,and F of 24.85%,15.28%and 16.97%,respectively.The validated method meets the requirements for biological sample analysis and is applicable for the pharmacokinetic evaluation of tiamulin formulations in minipigs.关键词
泰妙菌素/LC-MS/MS/小型猪/生物利用度/药代动力学Key words
tiamulin/LC-MS/MS/minipigs/bioavailability/pharmacokinetics分类
医药卫生引用本文复制引用
涂佳卉,耿梅,侯庆明,罗贤海,邱志霞,王聪..LC-MS/MS法测定小型猪血浆中泰妙菌素浓度及其在不同制剂药代动力学研究中的应用[J].中国药科大学学报,2026,57(1):54-59,6.
