中国药理学通报2026，Vol.42Issue(2)：332-340,9.DOI:10.12360/CPB202504099
β-细辛醚对缺血性脑卒中大鼠的作用
Effect of β-asarone on ischemic brain injury in rats
摘要
Abstract
Aim To investigate the therapeutic effects of β-asarone,a key active component of Acorus tatari-nowii,on rats with ischemic stroke.Methods Middle cerebral artery occlusion(MCAO)rats were es-tablished by using the intraluminal filament method and divided into the sham,model,β-asarone(various doses),and positive drug control groups.Treatments were administered for 7 d.The neurological deficit scores,Nissl staining for Nissl body morphology and density,NeuN protein,inflammatory cytokines were assessed.Network pharmacology was used to identify potential targets.To validate epidermal growth factor receptor(EGFR)involvement,the EGFR inhibitor was intracerebroventricularly injected 30 min before MCAO induction,followed by β-asarone treatment for 7 d.An in vitro oxygen-glucose deprivation/reoxygen-ation(OGD/R)model was established in PC12 cells.After β-asarone intervention,the cell viability,NeuN,EGFR protein levels,and inflammatory cytokines were measured by CCK-8 assay,Western blot and RT-qPCR,respectively.EGFR inhibitor was used to con-firm pathway specificity.Results β-asarone signifi-cantly improved neurological function in MCAO rats,increased Nissl body density and NeuN protein levels,and suppressed IL-1β,TNF-α,and IL-6 expression.In vitro,β-asarone protected PC12 cells against OGD/R-induced damage.Network pharmacology identified 121 potential targets for β-asarone in ischemic stroke,with enrichment analysis linking its mechanism to the EGFR signaling pathway.Western blot confirmed β-asarone upregulated EGFR.EGFR inhibition abol-ished β-asarone's neuroprotective effects.Conclu-sion β-asarone exerts protective effects against isch-emic stroke by activating the EGFR signaling pathway.关键词
β-细辛醚/缺血性脑卒中/NeuN/网络药理学/EGFR/石菖蒲Key words
β-asarone/ischemic stroke/NeuN/net-work pharmacology/EGFR/Acorus tatarinowii schott分类
医药卫生引用本文复制引用
刘威,叶淼,洪海棉,王翔锋,郑哲炀,李莹,赖文芳,林雅..β-细辛醚对缺血性脑卒中大鼠的作用[J].中国药理学通报,2026,42(2):332-340,9.基金项目
国家自然科学基金资助项目(No 82174001) （No 82174001）
福建省自然科学基金资助项目(No 2024J01761,2024J01776) （No 2024J01761,2024J01776）
