中国实用妇科与产科杂志2026，Vol.42Issue(1)：100-104,5.DOI:10.19538/j.fk2026010121
红核妇洁洗液对阴道加德纳菌及其生物膜作用的研究
Study on the effect of Honghe Fujie Lotion on gardnerella vaginalis and its biofilm
摘要
Abstract
Objective To investigate the effects of Honghe Fujie Lotion on Gardnerella vaginalis and its biofilm.Methods A total of 576 patients initially diagnosed with bacterial vaginosis at the Beijing Obstetrics and Gynecology Hospital,Capital Medical University,between November 2022 and June 2023 were enrolled.Ten strains of Gardnerella vaginalis were isolated,purified,and identified from their vaginal secretion samples.Honghe Fujie Lotion was co-cultured with Gardnerella vaginalis for 48 hours or applied to Gardnerella vaginalis pre-cultured for 24 hours.Parameters including OD value,inhibition rate,minimum inhibitory concentration(MIC),and minimum biofilm inhibitory concentration(MBIC)of Gardnerella vaginalis were measured.The status of vaginalis biofilm was observed by scanning electron microscopy.Results The biofilm formation capacity of Gardnerella vaginalis was the strongest after 48 hours of culture(P＜0.05).At the therapeutic dose(10％),the bactericidal rate exceeded 94％within 0-5 minutes,,which showed a rapid,strong and stable bactericidal effect of Honghe Fujie Lotion.Honghe Fujie Lotion could effectively inhibit the growth and biofilm formation of Gardnerella vaginalis even at a low concentration.The inhibitory rates for different strains were different.The MIC value of it ranged from 1/40 to 1/1280 and MBIC value was at 1/80 dilution,still significantly lower than the therapeutic dose.Under the scanning electron microscope,it was found that,even at a concentration lower than the therapeutic dose,Honghe Fujie Lotion could still exert a significant disruptive effect on Gardnerella vaginalis biofilm.Conclusion Honghe Fujie Lotion has a good bactericidal effect on Gardnerella vaginalis and can effectively inhibit the formation of biofilm and destroy the mature biofilm.关键词
红核妇洁洗液/细菌性阴道病/阴道加德纳菌/生物膜Key words
hongke fujie lotion/bacterial vaginosis/gardnerella vaginalis/biofilm分类
医药卫生引用本文复制引用
宗晓楠,赵晓雯,尚翔,白会会,范琳媛,刘朝晖..红核妇洁洗液对阴道加德纳菌及其生物膜作用的研究[J].中国实用妇科与产科杂志,2026,42(1):100-104,5.基金项目
国家自然科学基金(81771530) National Natural Science Foundation of China(81771530) （81771530）
