中华耳科学杂志2026，Vol.24Issue(2)：137-140,4.DOI:10.3969/j.issn.1672-2922.2026.02.008
小鼠听神经髓鞘的施万细胞分离纯化及巨噬细胞共培养研究
In vitro modeling of interaction between Schwann cells from auditory nerve myelin and macrophages
摘要
Abstract
Objective To establish an in vitro model to study the interaction between induced M1-type macrophages and auditory nerve myelin Schwann cells.Methods Cochlear tissues from 7-day-old C57BL/6J mice were selected,and nerve fragments were digested to produce a mixed culture of Schwann cells and fibroblasts.Purified Schwann cells were obtained using differential adhesion and cytosine arabinoside treatment.Mouse bone marrow monocytes were polarized into M1 macrophages using macrophage colony-stimulating factor and lipopolysaccharide.The purified Schwann cells were then co-cultured with the induced M1 macrophages at a 1:1 cell ratio.Analysis was performed using immunofluorescence staining,cell counting,and enzyme-linked immunosorbent assay.Results The results showed a significant decrease in the number of Schwann cells on the third day after co-culture compared to the first day(P<0.001).There was a statistically significant difference in the secretion levels of brain-derived neurotrophic factor in the supernatant between the pure Schwann cell culture group and the co-culture group(P<0.001).Conclusions This study successfully established,a co-culture model of auditory nerve myelin Schwann cells and macrophages.This method holds significant value for in-depth research on the mechanism of macrophage influence on auditory nerve myelin Schwann cells and provides a foundation for understanding the immune response in the inner ear.关键词
听神经髓鞘/耳蜗巨噬细胞/施万细胞/神经营养因子/共培养模型Key words
myelin auditory nerve/cochlear macrophages/Schwann cells/neurotrophic factor/co-culture model引用本文复制引用
张斯,柳柯,龚树生..小鼠听神经髓鞘的施万细胞分离纯化及巨噬细胞共培养研究[J].中华耳科学杂志,2026,24(2):137-140,4.基金项目
国家自然科学基金项目(82371151,82071037) （82371151,82071037）
北京市教育委员会科研计划项目资助(KZ20231002542) （KZ20231002542）
