中国饲料Issue(3)：46-57,12.DOI:10.15906/j.cnki.cn11-2975/s.2024100069-12
酶提及碱提法制备罗汉果多糖的工艺及体外降血糖与抗氧化活性研究
Preparation of polysaccharides from Siraitia grosvenorii by enzymatic and alkaline extraction and in vitro hypoglycemic and antioxidant activity studies
摘要
Abstract
The purpose of this study was to extracted polysaccharides from dried Siraitia grosvenorii using both enzymatic and alkaline extractions,and compared their polysaccharide profiles.Linvestigated the effects of enzyme activity,temperature,time,and pH in enzymatic extraction and alkaline concentration,temperature,time,and number of extractions in alkaline extraction,using the rate of polysaccharides of Siraitia grosvenorii as the evaluation criterion through the one-way test and orthogonal test,and optimized the preparation conditions.Meanwhile,their inhibitory effects on α-glucosidase,α-amylase and antioxidant effects were also investigated.The experimental results showed that in the enzyme extraction method,the optimal extraction conditions were enzyme activity of 3000 U,temperature of 60 ℃,time of 150 min,and pH of 5.In the alkaline extraction method,the optimal extraction conditions were alkali concentration of 1 mol/L,temperature of 40 ℃,time of 5 h,and the number of extraction times of 2.The alkaline extraction method yielded the highest extraction rate,which averaged 5.99％and was significantly higher than that of the enzyme extraction method(2.18％).The polysaccharides obtained by the two extraction methods did not differ much in total sugar and glucuronic acid content,and the solubility of the polysaccharides extracted by enzyme was the lowest.The alkali-extracted polysaccharides showed stronger activity in inhibiting α-glucosidase and α-amylase,which suggested their potential application in hypoglycemia.The Siraitia grosvenorii polysaccharides extracted in different ways showed better ability in scavenging DPPH radicals,hydroxyl radicals and superoxide anion,with obvious reducing ability,which indicated that Siraitia grosvenorii polysaccharides had better antioxidant activity.关键词
罗汉果多糖/酶提/碱提/降血糖/抗氧化Key words
Siraitia grosvenorii polysaccharide/enzyme extraction/alkaline extraction/hypoglycemic activity/antioxidant分类
农业科技引用本文复制引用
刘玉强,覃思思,董宝锐,李霞,姜铁民,关媛..酶提及碱提法制备罗汉果多糖的工艺及体外降血糖与抗氧化活性研究[J].中国饲料,2026,(3):46-57,12.基金项目
中央引导地方科技发展专项资金(桂科ZY22096025) （桂科ZY22096025）
广西科技计划项目(桂科AD20297088) （桂科AD20297088）
