中成药2026，Vol.48Issue(1)：33-41,9.DOI:10.3969/j.issn.1001-1528.2026.01.006
UPLC-Q-TOF-MS/MS法鉴定3种酚类在小鼠体内的代谢产物
Identification of in vivo metabolites of three phenols in mice by UPLC-Q-TOF-MS/MS
摘要
Abstract
AIM To establish a UPLC-Q-TOF-MS/MS method for the identification of in vivo metabolites of gallic acid,ellagic acid and corilagin in mice.METHODS The mice in administration groups were given intragastric administration of 0.5%CMC-Na suspensions of 3 constituents(400 mg/kg),respectively,while those in blank group were given intragastric administration of 0.5%CMC-Na suspensions(400 mg/kg),after which plasma,urine and feces were collected.The analysis was performed on a 30℃thermostatic Acquity UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm),with the mobile phase comprising of water(containing 0.1%formic acid)-acetonitrile flowing at 0.27 mL/min in a gradient elution manner,and electrospray ionization source was adopted in negative ion scanning.RESULTS Total 27 gallic acid metabolites,13 ellagic acid metabolites and 37 corilagin metabolites were identified,these 3 constituents mainly occured phase Ⅰmetabolites such as hydrolysis,decarboxylation,and phase Ⅱ metabolites such as methylation,sulfation,glucuronidation.CONCLUSION This stable and reliabe method can provide a reference for exploring the in vivo metabolic rules of other natural phenols.关键词
没食子酸/鞣花酸/柯里拉京/代谢产物/UPLC-Q-TOF-MS/MSKey words
gallic acid/ellagic acid/corilagin/metabolites/UPLC-Q-TOF-MS/MS分类
医药卫生引用本文复制引用
赵蓉,胡蒙蒙,洪玉冰,雷胜男,赵子康,车彦云..UPLC-Q-TOF-MS/MS法鉴定3种酚类在小鼠体内的代谢产物[J].中成药,2026,48(1):33-41,9.基金项目
兴滇英才支持计划项目(20220273) （20220273）
云南省基础研究计划项目面上项目(202401AT070160) （202401AT070160）
云南省科技厅-云南中医药大学应用基础联合研究专项项目面上项目(202101AZ070001-212) （202101AZ070001-212）
云南省科技厅-云南中医药大学应用基础联合研究专项项目重点项目(2019FF002-012) （2019FF002-012）
