中国药业2026，Vol.35Issue(4)：81-86,6.DOI:10.3969/j.issn.1006-4931.2026.04.014
白竭散抑制JAK2/STAT3信号通路促进肛瘘大鼠创面愈合
Baijie Powder Promoted Wound Healing in Rats with Anal Fistula via Inhibiting the JAK2/STAT3 Signaling Pathway
摘要
Abstract
Objective To investigate the effect of Baijie Powder on postoperative wound healing in rats with anal fistula.Methods The bacterial infection induction method was used to establish the anal fistula model rat.A total of 27 rats with successful modeling were randomly divided into the model group,the Baijie Powder group(1 g/cm2)and the Bovine Basic Fibroblast Growth Factor for External Use(Beifuji)group(150 IU/cm2),with 9 rats in each group,another 10 rats were selected as control group.After cleaning with normal saline,the corresponding drugs were sprayed on the wound,and fixed with sterile gauze,twice a day for two consecutive weeks.The wound healing in rats was observed,and the healing rate was calculated,and the wound edema was scored.Hematoxylin-eosin(HE)staining was used to observe the pathological morphology of granulation tissue in rat wound.Enzyme-linked immunosorbent assay(ELISA)method was used to detect the levels of interleukin-1 β(IL-1β),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),vascular endothelial growth factor(VEGF),angiotensin Ⅱ(Ang Ⅱ),platelet-derived growth factor(PDGF)and insulin-like growth factor 1 receptor(IGF-1R)of granulation tissue in rat wound.Immunofluorescence method was used to detect the apoptosis cells of granulation tissue in rat wound and calculate the apoptosis rate.qRT-PCR and Western blot method were used to detect the mRNA and protein expression levels of Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3)of granulation tissue in rat wound.Results Compared with the model group,the wound healing rates in the Baijie Powder group and Beifuji group were significantly increased(P<0.05);the wound edema scores were significantly decreased,the levels of IL-1β,TNF-α and IL-6 were significantly decreased(P<0.05);the levels of VEGF,Ang Ⅱ,PDGF and IGF-1R were significantly increased(P<0.05);the apoptosis rates of granulation tissue in wound were significantly decreased(P<0.05);the expression levels of JAK2 and STAT3 mRNA and protein were significantly decreased(P<0.05).Conclusion Baijie Powder can alleviate the inflammatory reaction of rats with anal fistula,promote angiogenesis,inhibit wound cell apoptosis,reduce wound edema,and promote wound healing.Its mechanism may be related to the inhibition of activation of JAK2/STAT3 signaling pathway.关键词
肛瘘/白竭散/创面愈合/炎性反应/Janus激酶 2/信号传导及转录激活因子 3 信号通路Key words
anal fistula/Baijie Powder/wound healing/inflammatory response/JAK2/STAT3 signaling pathway分类
医药卫生引用本文复制引用
陈伟特,周丽华,黄晓燕,林木南..白竭散抑制JAK2/STAT3信号通路促进肛瘘大鼠创面愈合[J].中国药业,2026,35(4):81-86,6.基金项目
福建医学科技青年培育项目[22FBQN2022059]. （）
