摘要
Abstract
Objective To establish a quantitative analysis of multi-components by single-marker(QAMS)method for simultaneous determination of prim-O-glucosylcimifugin,paeoniflorin,5-O-methylvisammioside,costunolide and dehydrocostus lactone in Jinshen Huashi Capsules.Methods The chromatographic column was BDS HypersilTM C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.05%phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 225 nm,the column temperature was 30 ℃,and the injection volume was 10 μL.Paeoniflorin was used as the internal standard to calculate the relative correction factor(RCF)of the other four components,and the content was determined by QAMS method.The reliability of the results was verified by the pearson correlation cofficient(r)between QAMS method and the external standard method.Results The linear range of the above five components were 5.58-55.81 μg/mL,18.78-187.83 μg/mL,7.39-73.91 μg/mL,4.78-47.81 μg/mL,4.99-49.98 μg/mL,respectively(r≥0.999 7,n=6),and the limit of detection was 0.15-0.63 μg/mL;the RSDs of precision,stability,and repeatability test results were all lower than 4.0%;the average recovery rate was 97.52%-99.22%,with RSDs of 0.72%-2.14%(n=6).The RCFs of prim-O-glucosylcimifugin,5-O-methylvisammioside,costunolide and dehydrocostus lactone relative to paeoniflorin were 0.491,0.491,0.796 and 0.946,respectively.The r of the above four components by the two methods were all≥0.997.Conclusion The established QAMS method can provide reference for the quality control of Jinshen Huashi Capsules.关键词
金参化湿胶囊/一测多评法/相对校正因子/多组分/定量测定Key words
Jinshen Huashi Capsule/quantitative analysis multi-components by single-marker/relative correction factor/multi-component/quantitative determination分类
医药卫生
