浙江医学2026，Vol.48Issue(2)：117-125,9.DOI:10.12056/j.issn.1006-2785.2026.48.2.2025-355
Notch信号通路激活状态与Th17/Treg失衡在胎儿宫内生长受限发病机制中的作用研究
Role of Notch signaling pathway activation and Th17/Treg imbalance in the pathogenesis of fetal intrauterine growth restriction
摘要
Abstract
Objective To investigate the role of notch homolog 1(Notch1)signaling pathway activation and T helper cell 17(Th17)/regulatory T cell(Treg)imbalance in the pathogenesis of fetal intrauterine growth restriction(IUGR).Methods A prospective study was conducted,enrolling 30 pregnant women with IUGR(IUGR group)treated at Jinhua Municipal Central Hospital from January 2023 to October 2024,along with 30 normal pregnant women in the third trimester(normal pregnancy group)and 30 healthy non-pregnant women(healthy non-pregnant group)during the same period.Blood samples were collected from all participants.Flow cytometry was used to detect the proportions of Th17 and Treg and CD4+Notch1+hairy and enhancer of split-1(Hes-1)+cells in peripheral blood.Enzyme-linked immunosorbent assay(ELISA)was employed to measure serum interleukin-17(IL-17)and transforming growth factor-β(TGF-β)levels.Western blot was used to detect Notch1 and Hes1 protein expression levels in peripheral blood.In the animal experiment,rats were divided into a control group,an IUGR group,and an IUGR+Notch inhibitor group(IUGR+inhitor group).Fetal and placental weights were measured,and flow cytometry,ELISA,and Western blot were used to assess the same indicators as in the human study.Results Compared with the normal pregnancy and healthy non-pregnant groups,the IUGR group showed significantly increased Th17 proportion,decreased Treg proportion,elevated Th17/Treg ratio,increased serum IL-17 levels,decreased TGF-β levels,and upregulated Notch1 and Hes1 protein expression levels(all P＜0.01).In the animal experiment,compared with the control group,the IUGR group exhibited reduced fetal and placental weights,increased Th 17 proportion,decreased Treg proportion,elevated Th17/Treg ratio,increased serum IL-17 levels,decreased TGF-β levels,and upregulated Notch1 and Hes1 protein expression levels(all P＜0.01).After Notch signaling pathway inhibitor intervention,compared with the IUGR group,the IUGR+inhibitor group showed increased fetal and placental weights,decreased Th17 proportion,increased Treg proportion,reduced Th17/Treg ratio,decreased serum IL-17 levels,increased TGF-β levels,and downregulated Notch1 and Hes1 protein expression levels(all P＜0.01).Conclusion Notch signaling pathway activation and Th 17/Treg imbalance play important roles in the pathogenesis of IUGR,providing potential targets for early diagnosis and treatment.关键词
胎儿宫内生长受限/Notch信号通路/辅助性T细胞17/调节性T细胞失衡Key words
Fetal intrauterine growth restriction/Notch signaling pathway/T helper cell 17/regulatory T cell imbalance引用本文复制引用
晏婷,袁里朝,施展华,吕英..Notch信号通路激活状态与Th17/Treg失衡在胎儿宫内生长受限发病机制中的作用研究[J].浙江医学,2026,48(2):117-125,9.基金项目
金华市科技计划项目(2021-3-124) （2021-3-124）
