中国中医眼科杂志2026，Vol.36Issue(2)：101-106,6.DOI:10.13444/j.cnki.zgzyykzz.2026.02.001
"二龙戏珠"针法对ONP大鼠眼外肌功能及动眼神经Galectin-1 mRNA表达的影响
Effect of the Erlong Xizhu Acupuncture Technique on Extraocular Muscle Function and Oculomotor Nerve Galectin-1 mRNA Expression in a Rat Model of Oculomotor Nerve Palsy
摘要
Abstract
OBJECTIVE To investigate the effects of the Erlong Xizhu acupuncture technique on extraocular muscle fun-ction and oculomotor nerve Galectin-1 mRNA expression in rats with oculomotor nerve palsy(ONP).METHODS A total of 50 rats were randomly assigned to the blank group(BG),sham-group(Sham),model group(MG),electroacupuncture group(EA),and Erlong Xizhu acupuncture group(ELXZ).The rats in the BG group received no intervention;The rats in the Sham group underwent surgical exposure of the oculomotor nerve without injury;The rats in the MG,EA,and ELXZ groups underwent exposure and clamping of the oculomotor nerve to establish the ONP model.Following successful modeling,the EA group received acupuncture at the superior rectus and inferior rectus muscles with electrical stimulation;The ELXZ group received Erlong Xizhu acupuncture once daily for 10 mins,six days as one course,for four consecutive courses.Before and after intervention,digital image analysis was used to measure pupil diameter and palpebral fissure height;a muscle tension transducer was employed to assess extraocular muscle tension;And quantitative real-time PCR(qRT-PCR)was performed to determine oculomotor nerve Galectin-1 mRNA expression.RESULTS(1)Pupil diameter:After intervention,pupil diameter decreased in EA and ELXZ groups compared with pre-intervention values.The MG group showed significantly larger pupil diameters than the BG and Sham groups,while EA and ELXZ groups had smaller pupil diameters than the MG group.The differences were all statistically significant(P<0.05).(2)Palpebral fissure height:After intervention,palpebral fissure height increased in EA and ELXZ groups.The MG group exhibited significantly reduced palpebral fissure height compared with BG and Sham groups,whereas EA and ELXZ groups had greater values than the MG group,with ELXZ higher than EA.The differences were all statistically significant(P<0.05).(3)Muscle tension:After intervention,extraocular muscle tension was elevated in EA and ELXZ groups compared with pre-intervention.The MG group had significantly lower muscle tension than the BG and Sham groups.The differences were all statistically significant(P<0.05).No significant difference was observed between EA and MG groups(P>0.05),whereas ELXZ showed significantly higher muscle tension than both MG and EA groups.The differences were all statistically significant(P<0.05).(4)Galectin-1 mRNA expression:The MG group had significantly reduced oculomotor nerve Galectin-1 mRNA expression compared with BG and Sham groups.Both EA and ELXZ groups exhibited significantly higher expression than the MG group.The differences were all statistically significant(P<0.05).No significant difference between EA and ELXZ groups(P>0.05).CONCLUSIONS The Erlong Xizhu acupuncture technique can improve extraocular muscle tension,reduce pupil diameter,enlarge palpebral fissure height,and increase Galectin-1 mRNA expression in the oculomotor nerve of ONP model rats.关键词
"二龙戏珠"针法/动眼神经麻痹/眼外肌/半乳糖凝集素-1Key words
Erlong Xizhu acupuncture/oculomotor nerve palsy/extraocular muscles/Galectin-1分类
医药卫生引用本文复制引用
曹水清,赵小鹏,赵峰,贾成文,文新,刘雪,宋亚文,张文晶.."二龙戏珠"针法对ONP大鼠眼外肌功能及动眼神经Galectin-1 mRNA表达的影响[J].中国中医眼科杂志,2026,36(2):101-106,6.基金项目
1甘肃省自然科学基金项目(21JR7RA584) 2甘肃省名中医(颉瑞萍)传承工作室建设项目 3甘肃中医药大学附属医院院内课题青年项目(gzfy-2022-16) （21JR7RA584）
