中国中医骨伤科杂志2026，Vol.34Issue(2)：28-34,7.DOI:10.20085/j.cnki.issn1005-0205.260204
山姜素抑制类风湿关节炎大鼠关节滑膜血管生成的机制研究
Mechanistic Study on Alpinetin-Mediated Suppression of Synovial Angiogenesis in Rheumatoid Arthritis Rats
摘要
Abstract
Objective:To investigate the effect of alpinetin on angiogenesis in rheumatoid arthritis(RA)rats by adjusting hypoxia-inducible factor-1α/vascular endothelial growth factor(HIF-1α/VEGF)pathway.Methods:The RA rat model was constructed using type II collagen induction method.The rats were separated into control group,arthritis model group,alpinetin treatment group,prednisolone treatment group,and alpinetin+cobalt chloride group.The arthritis index,joint swelling,and body weight of rats were measured to analyze clinical features.ELISA was used to measure the serum IL-17,IL-6,and IL-1β of rats in each group.HE staining reagent and safranin O-green were used to discuss the pathologi-cal features of ankle joints.Immunohistochemistry(IHC)and immunohistochemistry fluorescence(IHF)were used to detect the quantity of angiogenesis.Western Blot was used to detect the HIF-1α,VEGF,VEGFR,and CD31 proteins in synovial tissue.Results:Compared with the control group,the arthritis model group showed upregulation of arthritis index,joint swelling,IL-17 content,IL-6 content,IL-1β content,ankle HE score,safranin O score,CD31 positivity,number of new blood vessels,number of mature blood vessels,total blood vessels,HIF-1α protein,VEGF protein,VEGFR pro-tein,and CD31 protein,while downregulation of weight(P<0.05).Compared with the arthritis model group,the alpinetin treatment group and prednisolone treatment group showed downregulation of arthritis index,joint swelling,IL-17 content,IL-6 content,IL-1β content,ankle HE score,safra-nin O score,CD31 positivity,number of new blood vessels,HIF-1α protein,VEGF protein,VEGFR protein,and CD31 protein,while upregulation of weight(P<0.05).Compared with the alpinetin treatment group,the alpinetin+cobalt chloride group showed upregulation of arthritis index,joint swelling,IL-17 content,IL-6 content,IL-1β content,ankle HE score,safranin O score,CD31 positivity,number of new blood vessels,HIF-1α protein,VEGF protein,VEGFR protein,and CD31 protein,while downregulation of weight(P<0.05).The above indicators in the prednisolone treatment group were not prominently different(P>0.05).Conclusion:Alpinetin inhibits the HIF-1α/VEGF pathway and alleviates angiogenesis in RA rats.关键词
山姜素/缺氧诱导因子-1α/血管内皮生长因子通路/类风湿关节炎/血管生成Key words
alpinetin/hypoxia-inducible factor-1α/vascular endothelial growth factor pathway/rheumatoid arthritis/angiogenesis分类
医药卫生引用本文复制引用
王贺,刘佳,刘畅..山姜素抑制类风湿关节炎大鼠关节滑膜血管生成的机制研究[J].中国中医骨伤科杂志,2026,34(2):28-34,7.基金项目
河北省医学科学研究重点课题计划项目(20231838) （20231838）
