中药材2025，Vol.48Issue(8)：1892-1899,8.DOI:10.13863/j.issn1001-4454.2025.08.004
外源硒对灵芝主要农艺性状及代谢产物积累的影响
Effect of Exogenous Selenium on the Main Agronomic Traits and Metabolite Accumulation of Ganoderma lucidum
摘要
Abstract
Objective:To investigate the effects of different exogenous selenium sources on the growth characteristics of Ganoderma lucidum hyphae,the main agronomic traits of Ganoderma lucidum fruiting bodies and the accumulation characteristics of metabolites such as triterpenoids,sterols.Methods:Modified PDA,sawdust composite substrates and substitute cultivation substrates were used as the culture medium,and different concentrations of potassium selenocyanate(KSeCN)and sodium selenite(Na2 SeO3)were added to cultivate Ganoderma lucidum mycelium.The mycelium was cultured and the fruiting body of Ganoderma lucidum was grown.The growth rate of Ganoderma Lucidum mycelium and main agronomic traits of Ganoderma lucidum fruiting bodies were measured in different cul-ture media,as well as the contents of triterpenes,sterols,polysaccharides,proteins,amino acids in different parts of Ganoderma lucidum fruiting bodies.Results:The main agronomic traits of the mycelium of the tested strains and Ganoderma lucidum fruiting bodies were generally better in the KSeCN selenium source treatment group than in the Na2 SeO3 selenium source treatment group.The mycelium growth rate of strains CZ12,CZ22,CZ26 was faster,and the main agronomic traits of strain CZ22 fruiting bodies were the best.Under the cultiration concentration of 10 to 20 mg/kg KSeCN,the average contents of triterpenes and sterols,polysaccharides in fruiting bodies were 1.35%and 1.40%,respectively,which were increased by 18.42%and 12.00%compared with the control group.There were sig-nificant differences in the content of main metabolites in different parts of Ganoderma lucidum fruiting bodies of strain CZ26 under sele-nium rich cultivation,and the protein and amino acid contents in the fungal tube layer were the highest,with 24.53%and 16.30%,re-spectively.The shell layer had the highest content of triterpenes and sterols,with 1.49%.Conclusion:The main grgonomic traits of Gan-oderma Lucidum fruiting bodies of strain CZ22 under selenium-enriched cultivation are well expressed,the accumulation capacity of metabolic produets is strong,providing reference for efficient cultivation of Ganoderma lucidum with selenium.关键词
灵芝/硒/子实体/代谢产物Key words
Ganoderma lucidum(Leyss.ex Fr.)Karst./Selenium/Fruiting body/Metabolites分类
医药卫生引用本文复制引用
李学龙,史明文,李宏亮,宋艳雨,李超,张鹏..外源硒对灵芝主要农艺性状及代谢产物积累的影响[J].中药材,2025,48(8):1892-1899,8.基金项目
辽宁省农业科学院院长基金项目(2023MS0502) （2023MS0502）
辽宁省浑南区食药用菌科技特派团项目(2024020125-JH5/104) （2024020125-JH5/104）
辽宁省农业科学院食药用菌栽培学科项目(2019DD072311) （2019DD072311）
