植物遗传资源学报2026，Vol.27Issue(2)：318-327,10.DOI:10.13430/j.cnki.jpgr.20250916004
油菜萌发期耐渍性综合评价和种质筛选
Comprehensive Evaluation and Screening of Germplasm for Waterlogging Tolerance in Rapeseed(Brassica napus L.)at the Germination Stage
摘要
Abstract
Winter rapeseed(Brassica napus L.)cultivated in paddy fields frequently encounters waterlogging.To identify germplasm resources with strong waterlogging tolerance,353 accessions were evaluated under to a 12 h waterlogging treatment at the germination stage.The electrical conductivity of the soaking solution was immediately measured after treatmen.Six growth traits,i.e.,root length,shoot length,root fresh weight,shoot fresh weight,root dry weight,and shoot dry weight,were determined after a 7 d recovery.Membership function analysis,principal component analysis,and clustering were used together with unit electrical conductivity to evaluate waterlogging-tolerance of rapeseed germplasm and to identify waterlogging-tolerant resources at the germination stage.The results indicated that all six traits significantly decreased after waterlogging treatment during the germination stage of rapeseed.The relative values(treatment/control)for root length,root fresh weight,and root dry weight were significantly lower than those for the shoot traits.Relative root length,relative root fresh weight and relative root dry weight supplemented with unit electrical conductivity can be used for waterlogging tolerance evaluation of rapeseed at the germination stage according to the analysis using a stepwise regression model and the correlation between various traits and the D value.These accessions were classified into four distinct groups using the comprehensive evaluation index(D-value):extremely tolerant(D≥0.75,58 accessions),tolerant(0.54≤D≤0.74,111 accessions),sensitive(0.32≤D≤0.52,130 accessions),and extremely sensitive(D≤0.31,54 accessions).The similar four-tier classification were classified according to unit electrical conductivity(Uel):the extreme tolerant(Uel≤289 μS/(cm·g),32 accessions),tolerant(290.42 μS/(cm·g)≤Uel≤411.67 μS/(cm·g),146 accessions),sensitive(411.90 μS/(cm·g)≤Uel≤513.20 μS/(cm·g),110 accessions),and extremely sensitive(Uel≥515.03 μS/(cm·g),65 accessions).By integrating D-value(D≥0.95)and Uel(Ue1≤204.48 μS/(cm·g)),six germplasm resources,including Ningza 2009 and Chuanyou 83,were identified as the most waterlogging-tolerant rapeseed.These materials,which exhibit exceptional tolerance to waterlogging during the germination stage,can serve as a foundational resource for investigating waterlogging tolerance mechanisms in later stages of rapeseed development and for enhancing waterlogging-tolerant varieties.关键词
油菜/萌发期/耐渍性/种质资源/综合评价Key words
Brassica napus L./germination stage/waterlogging tolerance/germplasm resources/comprehensive evaluation引用本文复制引用
王才权,杨柳,刘婷婷,罗红森,肖黄宇杰,刘忠松..油菜萌发期耐渍性综合评价和种质筛选[J].植物遗传资源学报,2026,27(2):318-327,10.基金项目
国家自然科学基金(U23A20194)National Natural Science Foundation of China(U23A20194) （U23A20194）
