中国中药杂志2026，Vol.51Issue(4)：1080-1089,10.DOI:10.19540/j.cnki.cjcmm.20250831.401
黄芩-栀子药对通过调控SphK1介导的糖酵解缓解CIA小鼠骨侵蚀的机制研究
Mechanistic study on alleviation of bone erosion in CIA mice by Scutellariae Radix-Gardeniae Fructus drug pair via regulation of SphK1-mediated glycolysis
摘要
Abstract
This study aims to investigate whether Scutellariae Radix-Gardeniae Fructus(S-G)drug pairs alleviate joint inflammation and bone erosion in collagen-induced arthritis(CIA)mice by regulating sphingosine kinase 1(SphK1)-mediated glycolysis and inhibiting the differentiation of osteoclasts(OCs)induced by receptor activator of nuclear factor-κB ligand(RANKL).Potential therapeutic targets of S-G drug pairs were screened via network pharmacology,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment and molecular docking analyses.The effects of S-G drug pairs on bone erosion,inflammatory cytokines,glycolysis-related factors,and OCs differentiation were evaluated using both the CIA mouse model and the RAW264.7 cell in vitro induction system.In vivo results showed that S-G drug pairs alleviated joint swelling and synovial inflammation,reduced OCs number,and modulated the expression of key cytokines,including RANKL,osteoprotegerin(OPG),interleukin(IL)-6,and IL-10.In vitro findings further demonstrated that serum containing the drug significantly inhibited RANKL-induced OCs differentiation,reduced F-actin ring formation,and lowered levels of lactate acid(LA)and lactate dehydrogenase A(LDH-A).Additionally,it suppressed the expression of glycolysis-and bone resorption-related proteins and genes such as SphK1,pyruvate kinase M2(PKM2),oncoprotein c-Myc(c-Myc),and cathepsin K(CTSK),while also weakening the interaction between SphK1 and PKM2.In conclusion,S-G drug pairs could inhibit OCs differentiation by regulating SphK1-mediated glycolytic metabolism,thereby mitigating rheumatoid arthritis-induced bone erosion.关键词
黄芩-栀子药对/类风湿关节炎/破骨细胞/骨侵蚀/鞘氨醇激酶 1(SphK1)Key words
Scutellariae Radix-Gardeniae Fructus drug pair/rheumatoid arthritis/osteoclast/bone erosion/sphingosine kinase 1(SphK1)引用本文复制引用
李健健,甘珮荣,代继光,庞静,丁涛,吴虹..黄芩-栀子药对通过调控SphK1介导的糖酵解缓解CIA小鼠骨侵蚀的机制研究[J].中国中药杂志,2026,51(4):1080-1089,10.基金项目
国家自然科学基金面上项目(82374117) （82374117）
