中国中药杂志2026，Vol.51Issue(4)：1090-1098,9.DOI:10.19540/j.cnki.cjcmm.20251024.801
基于UPLC-Q-Exactive Orbitrap-MS和网络药理学结合动物实验探讨干姜拌捣五味子治疗痰饮哮喘的作用机制
Mechanism of Zingiberis Rhizoma mixed and triturated with Schisandrae Chinensis Fructus for treating phlegm asthma based on UPLC-Q-Exactive Orbitrap-MS and network pharmacology combined with animal experiments
摘要
Abstract
The effects of Zingiberis Rhizoma mixed and triturated with Schisandrae Chinensis Fructus(ZMTS)on phlegm asthma were investigated based on compositional analysis,network pharmacology,and animal experiments.The compositional analysis of ZMTS using UPLC-Q-Exactive Orbitrap-MS identified a total of 37 components,with 33 active components screened out by TCMSP and SwissADME.SwissTargetPrediction and Superpred were employed to search for targets corresponding to the active components,while GeneCards was used to search for targets of the active constituents,resulting in a total of 118 intersection targets.The protein-protein interaction(PPI)network of component-disease intersection targets was constructed using STRING 11.0 and visualized using Cytoscape 3.10.0.The DAVID platform was utilized to perform Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses on the intersection targets,revealing that the phosphatidylinositide 3-kinase/protein kinase B(PI3K/Akt)pathway was an important therapeutic pathway.A component-target-pathway network was constructed,and the core components and core targets were subjected to molecular docking,demonstrating a good binding effect between the core components and core targets.The mechanism of action of ZMTS was verified by establishing a mouse model of phlegm asthma through histopathological staining,immunohistochemistry(IHC),enzyme-linked immunosorbent assay(ELISA),and Western blot.The animal experiments showed that the high-dose ZMTS group could significantly improve the airway inflammatory response in the mice with phlegm asthma,reduce the levels of interleukin-17(IL-17)and interleukin-23(IL-23),and significantly inhibite the phosphorylation level of PI3K and Akt proteins.ZMTS exerts its effect in improving phlegm asthma by acting on targets such as IL-17 and IL-23 through multiple components,with the mechanism closely related to the regulation of PI3K/Akt signaling pathway,fully demonstrating the comprehensive effect of multi-components,multi-targets,and multi-pathways.关键词
干姜拌捣五味子/痰饮哮喘/UPLC-Q-Exactive Orbitrap-MS/网络药理学/孟河医派Key words
Zingiberis Rhizoma mixed and triturated with Schisandrae Chinensis Fructus/phlegm asthma/UPLC-Q-Exactive Orbi-trap-MS/network pharmacology/Menghe medical school引用本文复制引用
左玉婷,陈薇,黄玮,钟佩,刘雨欣,汤雯雯,陶文瀚,王晓丽,赵权,刘产明,金莹..基于UPLC-Q-Exactive Orbitrap-MS和网络药理学结合动物实验探讨干姜拌捣五味子治疗痰饮哮喘的作用机制[J].中国中药杂志,2026,51(4):1090-1098,9.基金项目
江苏省中医药科技发展计划项目(YB2020054) （YB2020054）
常州市"十四五"卫生健康高层次人才培养工程项目(2024CZBJ032) （2024CZBJ032）
常州市孟河医派传承创新项目(MH202431,MH202414) （MH202431,MH202414）
江苏省研究生科研与实践创新计划项目(SJCX25_0925,SJCX22_0870) （SJCX25_0925,SJCX22_0870）
