中国医科大学学报2026，Vol.55Issue(2)：127-132,6.DOI:10.12007/j.issn.0258-4646.2026.02.006
银杏叶提取物通过调控p38 MAPK/MLCK信号通路对高氧诱导的急性肺损伤小鼠肠黏膜屏障的保护作用
Effect of Ginkgo biloba extract on the intestinal mucosal barrier by regulating the p38 MAPK/MLCK signaling pathway in mice with hyperoxia-induced acute lung injury
摘要
Abstract
Objective To explore the effects of Ginkgo biloba extract(GBE)on the intestinal mucosal barrier in mice with hyperoxia-induced acute lung injury(ALI)via the p38 mitogen-activated protein kinase(MAPK)/myosin light chain kinase(MLCK)signaling pathway.Methods C57BL/6J mice were randomly divided into control,ALI,low-dose GBE,high-dose GBE,and high-dose GBE+anisomycin,with 10 mice in each group.Oxygen partial pressure(PaO2),carbon dioxide partial pressure(PaCO2),and the ratio of lung wet weight to dry weight(W/D)were measured.Histological staining was used to observe and score pathological changes in the lung and intestinal tissues.Serum inflammatory cytokines,D-lactic acid(D-LA),and intestinal sIgA levels were detected using an enzyme-linked immunosorbent assay.The expression of related proteins was assessed by Western blotting.Results Compared with the control group,the ALI group showed significantly decreased PaO2 and expression of occludin,claudin,and zonula occludens-1(ZO-1)proteins(P<0.05),while PaCO2,W/D,lung and intestinal pathological scores,inflammatory cytokines,D-LA,diamine oxidase(DAO),sIgA levels,and p-p38 MAPK/p38 MAPK and MLCK protein expression were significantly increased(P<0.05).Compared with the ALI group,the low-and high-dose GBE groups exhibited opposite changes,with more pronounced improvements in the high-dose GBE group.The high-dose GBE+anisomycin group showed significantly attenuated therapeutic effects compared to the high-dose GBE group(P<0.05).Conclusion GBE may protect the intestinal mucosal barrier by regulating the p38 MAPK/MLCK signaling pathway,reducing inflammation,and increasing the expression levels of intestinal mucosal barrier-related proteins.关键词
银杏叶提取物/高氧/急性肺损伤/肠黏膜屏障/p38 MAPK/MLCK/炎症Key words
Ginkgo biloba extract/hyperoxia/acute lung injury/intestinal mucosal barrier/p38 MAPK/MLCK/inflammation分类
医药卫生引用本文复制引用
何英,邢周雄,程云,耿争光,付豹,傅小云..银杏叶提取物通过调控p38 MAPK/MLCK信号通路对高氧诱导的急性肺损伤小鼠肠黏膜屏障的保护作用[J].中国医科大学学报,2026,55(2):127-132,6.基金项目
贵州省科技计划(黔科合支撑[2022]一般179) （黔科合支撑[2022]一般179）
遵义市科技计划(遵市科合HZ字(2023)207号) （遵市科合HZ字(2023）
