中国医科大学学报2026，Vol.55Issue(2)：139-145,7.DOI:10.12007/j.issn.0258-4646.2026.02.008
H2S代谢基因3-MPST和SQOR在肺纤维化中的作用及其机制
Roles and mechanisms of the H2S metabolism genes 3-MPST and SQOR in pulmonary fibrosis
摘要
Abstract
Objective To explore the roles and underlying mechanisms of hydrogen sulfide(H2S)-metabolizing genes 3-mercaptopyruvate sulfurtransferase(3-MPST)and sulfide quinone oxidoreductase(SQOR)in pulmonary fibrosis.Methods Forty Sprague-Dawley rats were randomly divided into the control and bleomycin-induced pulmonary fibrosis model groups,and lung tissues were sampled at 14 and 21 days post-treatment.The expression levels of 3-MPST and SQOR in the rat lungs were assessed via immunohistochemistry and quantitative real-time PCR.In vitro,A549 cells were divided into the control,TGF-β1,TGF-β1+NaHS,siRNA-3-MPST,siRNA-3-MPST+TGF-β1,siRNA-SQOR,and siRNA-SQOR+TGF-β1 groups.The expression levels of 3-MPST,SQOR,α-SMA,and E-cadherin were assessed via Western blotting and quantitative real-time PCR.The content of H2S and the level of reactive oxygen species(ROS)were also determined.Results The expression level of 3-MPST was significantly downregulated,whereas the expression level of SQOR was upregulated in the fibrotic lungs of rats in the model group compared with those in the control group(P<0.05).Compared with A549 cells in the control group,the A549 cells in the TGF-β1 group displayed decreased levels of 3-MPST,E-cadherin,and H2S,and increased levels of SQOR,α-SMA,and ROS(P<0.05).These effects were attenuated by NaHS in the TGF-β1+NaHS group compared with those in the TGF-β1 group.Compared with the TGF-β1 group,the levels of E-cadherin,SQOR,and H2S in the siRNA-3-MPST+TGF-β1 group were significantly decreased(P<0.05),while the levels of α-SMA and ROS were significantly increased(P<0.05).In the siRNA-SQOR+TGF-β1 group,the levels ofα-SMA,3-MPST,and ROS were significantly decreased(P<0.05),while the level of E-cadherin and H2S were significantly increased(P<0.05).Conclusion 3-MPST appears to inhibit EMT and pulmonary fibrosis by increasing H2S content and reducing ROS level,whereas SQOR may promote fibrosis by decreasing H2S content and increasing ROS level.NaHS can also inhibit EMT and pulmonary fibrosis.关键词
肺纤维化/上皮-间质转化/3-巯基丙酮酸硫转移酶/硫化物醌氧化还原酶/硫化氢代谢Key words
pulmonary fibrosis/epithelial-mesenchymal transition/3-mercaptopyruvate sulfurtransferase/sulfide quinone oxidoreduc-tase/hydrogen sulfide metabolism分类
医药卫生引用本文复制引用
李聪,付明霞,王楠,柏雪莲,刘乃国..H2S代谢基因3-MPST和SQOR在肺纤维化中的作用及其机制[J].中国医科大学学报,2026,55(2):139-145,7.基金项目
山东省自然科学基金(ZR2019MC019) （ZR2019MC019）
