中国医科大学学报2026，Vol.55Issue(2)：153-158,163,7.DOI:10.12007/j.issn.0258-4646.2026.02.010
苦参碱通过HMGB1/RAGE信号通路抑制人乳头瘤病毒阳性宫颈癌的发生和发展
Matrine inhibits the occurrence and development of human papilloma virus-positive cervical cancer by HMGB1/RAGE signaling pathway
摘要
Abstract
Objective To investigate the effects of matrine on the occurrence and development of human papilloma virus(HPV)-positive cervical cancer via regulation of the HMGB1/RAGE signaling pathway.Methods HeLa cells were randomly divided into control,matrine,pc-HMGB1,pc-NC,and matrine+pc-HMGB1 groups.CCK-8 and flow cytometry were used to detect cell viability and apoptosis.Transwell and cell scratch assays were performed to assess cell invasion and matrine expression,respectively.Three-di-mensional in vitro cultures were used to detect vascular mimicry(VM)in cells.Western blotting was used to detect the expression of HMGB1/RAGE signaling pathway proteins in the cells.We constructed HeLa xenograft nude mice and examined the effects of matrine on tumor growth,angiogenesis,and the expression of HMGB1/RAGE signaling pathway proteins.Results The cell viability,invasion number,matrine rate,lumen formation number,HMGB1 and RAGE protein expression in the matrine group were lower than those in the control group,and the apoptosis rate was increased(P<0.05).The trend of changes in various indicators in the pc-HMGB1 group was the opposite of that in the matrine group;the nude mouse experiment further confirmed matrine's inhibitory effect on the occurrence and development of cervical cancer cells.Pc-HMGB1 reversed the inhibitory effect of matrine on the occurrence and development of cervical cancer cells.Conclusion Matrine inhibits the development and progression of HPV-positive cervical cancer by blocking HMGB1/RAGE signaling pathway.关键词
苦参碱/HMGB1/RAGE/人乳头瘤病毒/宫颈癌/发生/发展Key words
matrine/HMGB1/RAGE/human papilloma virus/cervical cancer/occurrence/development分类
医药卫生引用本文复制引用
冉雪梦,侯政瑶,路瑶,吕佳..苦参碱通过HMGB1/RAGE信号通路抑制人乳头瘤病毒阳性宫颈癌的发生和发展[J].中国医科大学学报,2026,55(2):153-158,163,7.基金项目
山东省中医药科技项目(Z-2022007) （Z-2022007）
