中药新药与临床药理2026，Vol.37Issue(2)：323-331,9.DOI:10.19378/j.issn.1003-9783.2026.02.014
基于多指标成分定量联合化学计量学及熵权TOPSIS模型评价不同产地天葵子的质量
Quality Evaluation of Semiaquilegiae Radix from Different Origins Based on Multi-Component Quantification Combined with Chemometrics and Entropy Weight-TOPSIS Model
摘要
Abstract
Objective To establish a multi-component quantitative detection method for Semiaquilegiae Radix and evaluate the quality of samples from different origins by combining chemometrics and an entropy weight-TOPSIS model.Methods Using a Develosil ODS-MG-5 C18 column with acetonitrile-0.1％phosphoric acid as the mobile phase under gradient elution and wavelength switching(254,210 nm),an HPLC method was developed to simultaneously determine the contents of 11 components(thalictoside,lithospermoside,menisdaurin,griffonilide,ferulic acid,protocatechuic acid,magnoflorine,thalifendine,berberrubine,daucosterol,and β-sitosterol)in 18 batches of Semiaquilegiae Radix.The contents of alcohol-soluble extractives,total ash,and acid-insoluble ash were also measured.Cluster analysis(CA),principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA),and an entropy weight-TOPSIS model were employed to comprehensively evaluate the quality of Semiaquilegiae Radix from different origins.Results The contents of the 11 components in the 18 batches were as follows:thalictoside 0.692-1.185 mg·g-1,lithospermoside 1.824-3.132 mg·g-1,menisdaurin 0.531-1.078 mg·g-1,griffonilide 0.223-0.544 mg·g-1,ferulic acid 0.202-0.392 mg·g-1,protocatechuic acid 0.987-1.948 mg·g-1,magnoflorine 0.825-2.212 mg·g-1,thalifendine 0.380-0.812 mg·g-1,berberrubine 0.112-0.328 mg·g-1,daucosterol 0.050-0.104 mg·g-1,and β-sitosterol 0.349-0.558 mg·g-1.The contents of alcohol-soluble extractives,total ash,and acid-insoluble ash were 12.495％～20.139％,3.125％～6.477％,and 1.120％～3.543％,respectively.CA and PCA results indicated that the 18 batches could be classified into three categories:S1-S7,S8-S12,and S13-S18.The OPLS-DA model screened five markers with VIP values>1 that significantly influenced quality variation:lithospermoside,magnoflorine,protocatechuic acid,menisdaurin,and griffonilide.The entropy weight-TOPSIS model results showed that the Euclidean closeness degree to the optimal solution for the 18 samples ranged from 0.130 7 to 0.772 7,indicating quality differences among Semiaquilegiae Radix from different origins.Conclusion The established multi-component quantification method is accurate and reliable.Combined with chemometrics and the entropy weight-TOPSIS model,it can effectively evaluate the quality of Semiaquilegiae Radix from different origins,providing a valuable reference for its quality control and comprehensive assessment.关键词
天葵子/多指标成分定量/高效液相色谱法/化学计量学/熵权TOPSIS模型/质量评价Key words
Semiaquilegiae Radix/multi-component quantification/HPLC/chemometrics/entropy weight-TOPSIS model/quality evaluation分类
医药卫生引用本文复制引用
迟静,贺玲,毕夏,祝丽超..基于多指标成分定量联合化学计量学及熵权TOPSIS模型评价不同产地天葵子的质量[J].中药新药与临床药理,2026,37(2):323-331,9.基金项目
辽宁省教育厅高等学校基本科研面上项目(LJKZ0893) （LJKZ0893）
辽宁省中药传承技术骨干人才培养项目(辽中医药综合字[2021]70号) （辽中医药综合字[2021]70号）
辽宁省中医药特色人才培养项目(辽中医药综合字[2021]38号). （辽中医药综合字[2021]38号）
