中药材2025，Vol.48Issue(9)：2185-2188,4.DOI:10.13863/j.issn1001-4454.2025.09.008
应用环介导等温扩增技术鉴别毛慈菇与冰球子
Identification of Cremastra appendiculata and Pleione bulbocodioides Using Loop-Mediated Isothermal Amplification Technology
摘要
Abstract
Objective:To establish a specific loop-mediated isothermal amplification(LAMP)method system for the rapid identifi-cation of two Chinese herbal medicines,Cremastra appendiculata and Pleione bulbocodioides.Methods:The rbcL coding region fragment in the chloroplast fragment was selected as the detection target.Specific primers for the identification of Cremastra appendiculata and Pleione bulbocodioides were designed.The LAMP identification method was established,and its methodological indicators such as speci-ficity,sensitivity and applicability were investigated.Results:The established LAMP rapid detection method under the reaction tempera-ture of 65℃and reaction time of 60 min could effectively distinguish the two easily-confused Chinese medicinal materials Cremastra appendiculata and Pleione bulbocodioides.The detection sensitivity could reach 500 ag/μL,and the reaction results were easy to ob-serve.Conclusion:The LAMP rapid identification method for the Chinese medicinal materials Cremastra appendiculata and Pleione bul-bocodioides established in this study has the characteristics of high sensitivity,good specificity,rapidity and practicality,and easy promo-tion.It is suitable for the quality monitoring of two Chinese medicinal materials Cremastra appendiculata and Pleione bulbocodioides.关键词
毛慈菇/冰球子/环介导等温扩增(LAMP)/rbcL基因/快速鉴定Key words
Cremastra appendiculata(D.Don)Makino/Pleione bulbocodioides(Franch.)Rolfe/Loop-mediated isothermal ampli-fication(LAMP)/rbcL Gene/Rapid identification分类
医药卫生引用本文复制引用
李杰,薛雅娟,王莉芳,寇娟,贾首前,张鹏..应用环介导等温扩增技术鉴别毛慈菇与冰球子[J].中药材,2025,48(9):2185-2188,4.基金项目
陕西省自然科学基础研究计划项目(2021JM-596) （2021JM-596）
