中药材2025，Vol.48Issue(9)：2189-2193,5.DOI:10.13863/j.issn1001-4454.2025.09.009
维西糯山药遗传多样性分析及指纹编码构建
Analysis of Genetic Diversity and DNA Fingerprint Construction of Dioscorea opposita from Weixi Lisu Autonomous County
摘要
Abstract
Objective:To determine the genetic relationship between Dioscorea opposita from Weixi Lisu Autonomous County and other germplasms,to construct the DNA fingerprint map and QR code of Dioscorea opposita from Weixi Lisu Autonomous County,so as to provide a scientific basis for protecting the characteristic varieties of Dioscorea opposita from Weixi Lisu Autonomous County.Meth-ods:By using the SSR molecular marker method,the DNA samples of 40 Dioscorea opposita germplasms were amplified to analyze the genetic diversity characteristics.The DNA fingerprint map and QR code of the germplasm resources of Dioscorea opposita from Weixi Lisu Autonomous County were constructed by using software such as NTSYS2.0e and Excel.Results:Among the 23 pairs of candidate SSR primers,6 pairs of amplitation-stable SSR primers were finally selected.The genetic diversity of Dioscorea opposita from Weixi Lisu Autonomous County was obvious.Among them,the variation of the Dioscorea opposita from Weixi Lisu Autonomous County grown in Kangpu Township was particularly prominent.The results of cluster analysis showed that when the similarity coefficient was 0.28,40 Di-oscorea opposita germplasm resources could be classified into three major categories.Conclusion:The Dioscorea opposita from Weixi Lisu Autonomous County in Baohe Town and Baijixun Township has been recognized as a major high-quality cultivated variety.Based on this,this study constructs the DNA fingerprint map and QR code of Dioscorea opposita from Weixi Lisu Autonomous County,laying a theoretical basis and technical support for the identification of Dioscorea opposita from Weixi Lisu Autonomous County germplasm re-sources and the breeding of superior varieties.关键词
维西糯山药/SSR分子标记/DNA指纹图谱/遗传多样性分析Key words
Dioscorea opposita Thunb.from Weixi Lisu Autonomous County/SSR molecular marker technique/DNA fingerprint-ing/Genetic diversity analysis分类
医药卫生引用本文复制引用
施健忠,白兴荣,杜彩艳,杨鹏,张永红,彭慧娉,蜂述先..维西糯山药遗传多样性分析及指纹编码构建[J].中药材,2025,48(9):2189-2193,5.基金项目
维西县乡村振兴涉农项目(ZD20210147) （ZD20210147）
云南省科技创新强省计划项目(2014AE014) （2014AE014）
云南省科技厅"三区"科技人才支持计划项目(2023) （2023）
