中药材2025，Vol.48Issue(9)：2194-2200,7.DOI:10.13863/j.issn1001-4454.2025.09.010
开心散基准样品多指标成分的薄层色谱鉴定
Thin-Layer Chromatography Identification of Multi-Index Components in Kaixinsan Reference Samples
摘要
Abstract
Objective:To establish a thin layer chromatography(TLC)method for the efficient identification of multiple index com-ponents in the reference samples of the classic prescription"Kaixinsan".Methods:This study systematically investigated the effects of major factors such as the preparation of the test sample,the composition of the developing agent,the developing environment(tempera-ture and humidity),and thin-layer chromatography plates from different manufacturers on the thin-layer chromatography behavior of six indicator components,namely ginsenoside Rb1,ginsenoside Re,tenuifolin,β-asarone,dehydrotumulosic acid and pachymic acid,in the Kaixinsan reference sample.And it was verified through actual measurement of samples.Results:The established thin-layer chromatogra-phy method effectively isolated and identified six indicator components in Kaixinsan.The spots of the six indicator components were clearly colored,with good separation,high reproducibility,negative without interference,and good durability.Conclusion:The thin-layer chromatography identification method for simultaneous detection of multiple standard components in the Kaixinsan reference sample es-tablished by this research institute has strong specificity and applicability,providing necessary experimental basis for the subsequent re-search and formulation of Kaixinsan quality standards.关键词
开心散/基准样品/薄层鉴别/质量标准/指标性成分Key words
Kaixinsan/Reference sample/Thin layer chromatographic/Quality standard/Indicative components分类
医药卫生引用本文复制引用
陶稳稳,李大伟,张彩云,金凤华,汝天乐,年立忠,黄顺旺..开心散基准样品多指标成分的薄层色谱鉴定[J].中药材,2025,48(9):2194-2200,7.基金项目
安徽省科技重大专项项目(202203a07020031) （202203a07020031）
安徽省自然科学基金项目(2408085MH229) （2408085MH229）
