针灸推拿医学（英文版）2026，Vol.24Issue(1)：1-13,13.DOI:10.1007/s11726-026-1544-2
艾灸对克罗恩病大鼠结肠H3K27乙酰化的调节作用研究
Study on the regulation effect of moxibustion on colonic H3K27 acetylation in rats with Crohn disease
摘要
Abstract
Objective:To investigate the regulatory effect of moxibustion on rats with Crohn disease(CD)and explore the relationship between the effect and alterations in colonic histone H3 lysine 27 acetylation(H3K27ac). Methods:Ten out of 36 Sprague-Dawley rats were randomly selected as the control group using a random number table method.The remaining 26 rats received an enema of 2,4,6-trinitrobenzene sulfonic acid to establish a CD model.Successfully modeled rats were then randomly divided into a model group,an herb-insulated moxibustion group,and a sulfasalazine group using the random number table method,with 8 rats in each group.The herb-insulated moxibustion group received herb-insulated moxibustion at Qihai(CV6)and bilateral Tianshu(ST25),and the sulfasalazine group received sulfasalazine via gavage.Histopathological changes in the colonic tissues were assessed using hematoxylin-eosin staining.The serum levels of inflammatory factors[tumor necrosis factor(TNF)-α,interleukin(IL)-1β,and IL-6]were measured using the enzyme-linked immunosorbent assay.The protein expression of colonic H3K27ac was detected using Western blotting and immunohistochemistry.The mRNA and protein expression levels of K-acetyltransferase 2B(KAT2B),general control non-repressed protein 5(GCN5),histone deacetylase 1(HDAC1),and histone deacetylase 3(HDAC3)in the colon were determined using real-time quantitative reverse transcription polymerase chain reaction and immunofluorescence,respectively. Results:Compared to the control group,the histopathological score,serum levels of TNF-α,IL-1β,and IL-6,and the mRNA and protein expression of GCN5,HDAC1,and HDAC3 in the colon were significantly increased in the model group(P<0.01 or P<0.05),whereas the protein expression of H3K27ac and the mRNA and protein expression of KAT2B were significantly decreased in the model group(P<0.01 or P<0.05).In contrast,compared to the model group,the histopathological score,serum levels of TNF-α,IL-1β,and IL-6,and the mRNA and protein expression of GCN5,HDAC1,and HDAC3 in the colon were significantly decreased in the herb-insulated moxibustion group(P<0.01 or P<0.05),whereas the protein expression of H3K27ac and the mRNA and protein expression of KAT2B were significantly increased in the model group(P<0.01 or P<0.05).No significant differences in the aforementioned indicators were observed between the sulfasalazine group and the herb-insulated moxibustion group. Conclusion:Moxibustion alleviates colonic inflammation in CD rats,potentially by up-regulating the expression of KAT2B,down-regulating the expression of GCN5,HDAC1,and HDAC3,and consequently increasing the H3K27ac level.关键词
灸法/药饼灸疗法/穴,天枢/穴,气海/克罗恩病/组蛋白乙酰转移酶/组蛋白去乙酰化酶/大鼠Key words
Moxibustion Therapy/Herb-insulated Moxibustion/Point,Tianshu(ST25)/Point,Qihai(CV6)/Crohn Disease/Histone Acetyltransferases/Histone Deacetylases/Rats分类
医药卫生引用本文复制引用
祁琴,吴璐一,邓指辉,马喆,陈立铭,吴焕淦,林亚莹,李国娜,郑寒丹,朱璐,钟蕊,黄艳..艾灸对克罗恩病大鼠结肠H3K27乙酰化的调节作用研究[J].针灸推拿医学（英文版）,2026,24(1):1-13,13.基金项目
This work was supported by the Project of National Natural Science Foundation of China(国家自然科学基金项目,No.82205262) （国家自然科学基金项目,No.82205262）
National Natural Science Foundation of China-Excellent Young Scientists Fund(国家自然科学基金优秀青年基金项目,No.82422074) （国家自然科学基金优秀青年基金项目,No.82422074）
Shanghai Sailing Program(上海市科学技术委员会扬帆专 项,No.22YF1444100) （上海市科学技术委员会扬帆专 项,No.22YF1444100）
Health Industry Clinical Research Special Projects of Shanghai Municipal Health Commission(上海市卫生健康委员会卫生行业临床研究专项项目,No.20244Y0150,No.20254Y0043) （上海市卫生健康委员会卫生行业临床研究专项项目,No.20244Y0150,No.20254Y0043）
Shanghai University of Traditional Chinese Medicine Science and Technology Development Project(上海中医药大学科技发展项目,No.24KFL076). （上海中医药大学科技发展项目,No.24KFL076）
