中药材2025，Vol.48Issue(7)：1645-1650,6.DOI:10.13863/j.issn1001-4454.2025.07.007
特异性PCR法鉴别白僵菌及其混伪品
Identification of Beauveria bassiana and Its Mixed Counterfeits by Specific PCR Method
摘要
Abstract
Objective:To establish a specific polymerase chain reaction(PCR)method for the rapid identification of Bombyx Ba-tryticatus and its adulterants.Methods:The ITS sequence of Beauveria bassiana,a fungal species associated with Bombyx Batryticatus,was compared with the corresponding gene sequences of related species.The specific primer TF2/TR2 was designed based on the frag-ments with a high number variation sites,and the PCR reaction conditions were optimized and validated.Results:At an annealing tem-perature of 64℃and with 30 cycles,all 14 batches of Bombyx Batryticatus,the powdered medicinal materials containing Bombyx Ba-tryticatus,and standard samples of Beauveria bassiana yielded a single clear target band at 258 bp.In contrast,none of the 27 batches of adulterants displayed this band.Conclusion:The specific PCR method established in this research institute accurately realizes the au-thenticity identification of Bombyx Batryticatus and its adulterants.This method has good specificity,is convenient and fast to operate,and is suitable for the quality control of Bombyx Batryticatus,providing technical support for addressing the issues of the stability and safety of Bombyx Batryticatus medicinal material.关键词
僵蚕/白僵菌/特异性引物/聚合酶链式反应Key words
Bombyx Batryticatus/Beauveria bassiana(Bals.)Vuillant/Specific primers/Polymerase chain reaction分类
医药卫生引用本文复制引用
贺媛,陈佳悦,钱正明,金李玲,黄琦,李文庆..特异性PCR法鉴别白僵菌及其混伪品[J].中药材,2025,48(7):1645-1650,6.基金项目
2023年度湖北省自然科学基金中医药创新发展联合基金项目(2023AFD157) （2023AFD157）
