中药材2025，Vol.48Issue(7)：1651-1656,6.DOI:10.13863/j.issn1001-4454.2025.07.008
美丽胡枝子及其同属混淆品的DNA条形码鉴定
DNA Barcoding Identification of Lespedeza formosa and Its Fakes from the Same Genus
摘要
Abstract
Objective:To conduct molecular identification of Lespedeza formosa and its mixed products by using DNA barcoding technology,in order to ensure the stable and reliable quality of clinical medication.Methods:A total of 27 samples of Lespedeza formosa from different origins and their similar easily mixed varieties were collected.The gene sequences of ITS2,psbA-trnH,matK and rbcL were used as candidate sequences for identification.Through PCR amplification and sequencing,the sequencing success rates and sequence characteristics of different candidate sequences were compared and evaluated,and the variations and genetic distances within and be-tween species were calculated.The phylogenetic tree was constructed.Results:Except for the matK sequence,the sequencing success rates of ITS2,rbcL,psbA-trnH were 100%,81.5%,66.7%,respectively.The ITS2 amplified sequence had more differential variation sites than the rbcL sequence,and some base variations could be used as identification features.Conclusion:The ITS2 sequence can ef-fectively distinguish the Lespedeza formosa and its mixed products,and can be used as the preferred identification sequence for DNA barcoding.This result provides a reference basis for enriching and improving the technical means of species authenticity identification of Lespedeza formosa and formulating relevant medicinal material quality standards.关键词
美丽胡枝子/DNA条形码/分子鉴定/ITS2Key words
Lespedeza formosa(Vogel)Koehne/DNA barcode/Molecular identification/ITS2分类
医药卫生引用本文复制引用
覃静,李州炜,李想,刘亚丽,黎理,傅静,王孝勋..美丽胡枝子及其同属混淆品的DNA条形码鉴定[J].中药材,2025,48(7):1651-1656,6.基金项目
广西科技基地和人才专项(桂科AD21238031) （桂科AD21238031）
广西壮瑶药重点实验室项目(桂科基字[2014]32号) （桂科基字[2014]32号）
广西重点研发计划项目(桂科AB21196016) （桂科AB21196016）
广西中医药重点学科壮药学(GZXK-Z-20-64) （GZXK-Z-20-64）
广西一流学科中药学(民族药学)(桂教科研[2018]12号) （民族药学）
