中国水产科学2025，Vol.32Issue(12)：1734-1748,15.DOI:10.12264/JFSC2025-0198
浅棕条鳅胚胎发育与早期发育特征
Developmental characteristics of embryos and early larvae of Schistura scaturigina
摘要
Abstract
Schistura scaturigina,endemic to the lower reaches of the Yarlung Zangbo River,holds considerable ecological and economic value.In this study,we presented the first systematic report on its embryonic and early larval development under artificial breeding conditions to clarify its developmental chronology and characteristics.These findings fill a critical research gap and provide important implications for the conservation and sustainable utilization of this species.We conducted experiments in April 2024.Hormonal induction was performed using a mixture of luteinizing hormone-releasing hormone analog(LHRH-A2),human chorionic gonadotropin(HCG),and domperidone(DOM).Females received a two-injection protocol:the first injection consisted of LHRH-A2(3 μg/kg)+DOM(2 mg/kg),followed 12 h later by a second injection of LHRH-A2(10 μg/kg)+DOM(8 mg/kg)+HCG(2000 IU/kg).Males received a single injection at the time of the female's second injection,at half the female dosage.Approximately 2,000 fertilized eggs were obtained via artificial dry fertilization,yielding average fertilization and hatching rates of 95.60％and 90.40％,respectively.Over 1,700 healthy larvae were ultimately obtained.The results showed that mature eggs were spherical,light yellow,and lustrous,with slight adhesiveness and a tendency to sink upon contact with water.Egg diameter measured 1.08±0.03 mm,expanding to(2.34±0.08)mm after water absorption.At a water temperature of(20.0±1.0)℃,fertilized eggs completed intra-membrane hatching in 49 h 20 min,requiring an accumulated temperature of 986.65 h·℃.Embryonic development was divided into 33 phases across seven stages:fertilized egg,cleavage,blastula,gastrula,neurula,organogenesis,and hatching.Newly hatched larvae measured(4.18±0.30)mm in total length,exhibiting transparent bodies and no pigmentation on the eyes or skin.Distinct pigmentation appeared across the body at 2 days post-hatching.The yolk sac was nearly absorbed at 5 days post-hatching,coinciding with the development of an oval,vacuole-like,and membranous swim bladder.At 10 days post-hatching,a pair of small spherical bony swim bladders had formed,exhibiting a dumbbell-like shape.Functional organs were essentially fully developed at 15 days post-hatching.Overall growth and development of early larvae demonstrated an increasing trend,albeit with stage-specific variations in developmental rate.Our findings reveal that while embryonic developmental chronology of S.scaturigina aligns with that of phylogenetically close species,specific traits observed in its organogenesis progression and newly hatched larval morphology may reflect adaptations to the unique torrential environment of the lower Yarlung Zangbo River.Given its current restricted distribution,enhanced management within protected areas is recommended.Future efforts should prioritize systematic resource surveys and long-term monitoring to provide a scientific foundation for basin-wide conservation strategies.关键词
浅棕条鳅/人工繁育/胚胎发育/仔鱼Key words
Schistura scaturigina/artificial breeding/embryonic development/fish larvae分类
农业科技引用本文复制引用
杨培灿,张志明,苏奇,贺凯,张驰,隋丽英..浅棕条鳅胚胎发育与早期发育特征[J].中国水产科学,2025,32(12):1734-1748,15.基金项目
国家重点研发计划项目(2024YFD1200703) （2024YFD1200703）
西南地区重点水域渔业资源与环境调查专项(CJW2023034) （CJW2023034）
西藏自治区重点科技计划项目(XZ202301ZY0012N). （XZ202301ZY0012N）
