中国水产科学2025，Vol.32Issue(12)：1749-1762,14.DOI:10.12264/JFSC2025-0252
急性操作胁迫对黄条鰤血清生化指标、抗氧化能力、鳃组织形态与体表黏液菌群的影响
Effects of acute handling stress on serum biochemical parameters,antioxidant capacity,gill morphology,and surface-slime microbial community of Seriola aureovittat
摘要
Abstract
In this study,we investigated the effects of acute handling stress on serum biochemical indicators,antioxidant capacity,gill morphology,and surface slime microbial community of Seriola aureovittata.The experiment was conducted using S.aureovittata individuals(body weight:463.86±63.86 g).During the experiment,crowding-air exposure was repeated 10 times.Samples were collected before acute handling stress(C0,control group)and at 0,3,6,12,and 24 h after stress.The results showed that acute handling stress significantly increased serum cortisol levels(P<0.05).With the extended recovery time,cortisol levels initially increased and subsequently decreased,peaking at 3 h and returning to baseline at 12 h.Superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)activities and malondialdehyde(MDA)content followed a similar trend to that of cortisol;however,they peaked at 6 h after handling stress.After 24 h of recovery,only CAT activity and MDA content returned to their initial levels.In addition,acute handling stress caused bending and swelling of gill lamellae,cell vacuolation,and disordered epithelial arrangement.After 24 h of recovery,gill filament morphology had not fully returned to its original state.Moreover,acute handling stress exacerbated slime microbiota dysbiosis on the body surface,significantly increasing pathogenic bacteria abundance,including Vibrio splendidus,Tenacibaculum soleae,and Tenacibaculum dicentraci(P<0.05).In conclusion,S.aureovittata requires more than 24 h to fully recover from acute handling stress.The optimal window for stress-alleviating interventions occurs within the first 6 h post-handling stress.Regulating slime microbiota balance on the body surface may represent a promising strategy for mitigating acute handling stress.关键词
黄条鰤/急性操作胁迫/抗氧化能力/黏液菌群/血清生化指标Key words
Seriola aureovittata/acute handling stress/antioxidant capacity/slime microbiota/serum biochemical parameter分类
农业科技引用本文复制引用
郑纪昌,冯元,徐永江,崔爱君,姜燕,王正浩..急性操作胁迫对黄条鰤血清生化指标、抗氧化能力、鳃组织形态与体表黏液菌群的影响[J].中国水产科学,2025,32(12):1749-1762,14.基金项目
国家重点研发计划项目(2022YFD2401102) （2022YFD2401102）
"泰山产业领军人才工程"项目 （）
山东省重点研发计划项目(2023TZXD050) （2023TZXD050）
青岛市海洋科技创新专项(24-1-3-hygg-22-hy) （24-1-3-hygg-22-hy）
中国水产科学研究院基本科研业务费项目(2023TD51,2024XT0701) （2023TD51,2024XT0701）
国家海水鱼产业技术体系项目(CARS-47). （CARS-47）
