中国水产科学2025，Vol.32Issue(12)：1775-1785,11.DOI:10.12264/JFSC2025-0133
长吻鮠幼鱼饲料中混合植物蛋白替代鱼粉的适宜比例研究
Dietary replacement of fishmeal with mixed vegetable proteins in juvenile Chinese longsnout catfish(Leiocassis longirostris)
摘要
Abstract
In recent years,fishmeal supply has surpassed market demand,leading to consistently high prices.To alleviate market pressure and reduce farming costs,it is crucial to investigate economical plant protein alternatives in feed.In this study,we examined the feasibility of incorporating mixed plant proteins into diets for juvenile Chinese longsnout catfish and determined appropriate inclusion levels.Six isonitrogenous and isolipidic diets were formulated using a mixed plant protein source(fermented soybean meal to rapeseed meal ratio of 4:1)to replace fishmeal in a basal diet at levels of 0％,10％,20％,30％,40％,and 50％(V0,V10,V20,V30,V40,and V50,respectively).Juvenile catfish were fed these diets for 8 weeks,after which growth performance,serum biochemistry,liver antioxidant activity,lipid content,and liver morphology were assessed.The results showed that substituting 50％of dietary protein with vegetable protein sources significantly reduced(P<0.05)the specific growth rate(SGR),whereas the V50 group exhibited a significantly higher feed conversion ratio that of other groups(P<0.05).Blood total cholesterol initially decreased and subsequently increased,reaching its lowest level in V10(P<0.05).Similarly,both aspartate aminotransferase and alanine aminotransferase displayed decreasing-then-increasing trends,with lowest levels in V30 and V20,respectively(P<0.05).Liver malondialdehyde showed a decreasing-then-increasing trend,with significantly lower values in V10 and V20 groups than those of other groups(P<0.05).Total antioxidant capacity demonstrated an increasing-then-decreasing pattern,peaking at V10.Catalase activity showed a decreasing trend,with the V40 group showing significantly higher activity than that of the V50 group but lower than that of other groups(P<0.05).Histological analysis revealed progressive hepatic steatosis beginning at V30,accompanied by blurred hepatocyte membrane boundaries.Lipid droplet quantity exhibited a decreasing-then-increasing trend,reaching its lowest level at V20(P<0.05).Under experimental conditions,moderate mixed vegetable protein supplementation improved juvenile Chinese longsnout catfish liver health.SGR was achieved with 37.47％fishmeal replacement by mixed vegetable protein;however,based on liver health indicators,a lower replacement level may be warranted.关键词
混合植物蛋白/长吻鮠/生长性能/肝脏抗氧化/脂肪蓄积/鱼粉替代Key words
mixed vegetable proteins/Leiocassis longirostris/growth performance/hepatic antioxidants/lipid accumulation/replacement of fish meal分类
农业科技引用本文复制引用
李建蔚,朱文欢,胡文广,张孝睿,黄天乐,陈见,谭青松..长吻鮠幼鱼饲料中混合植物蛋白替代鱼粉的适宜比例研究[J].中国水产科学,2025,32(12):1775-1785,11.基金项目
国家自然科学基金面上项目(32072950) （32072950）
武汉生物育种关键技术攻关及新品种培育科技重大专项(2022021302024854). （2022021302024854）
