中国水产科学2025，Vol.32Issue(12)：1798-1807,10.DOI:10.12264/JFSC2025-0054
室内卤虫高密度养殖投喂策略及卤虫形态变化关键期探究
Study of feeding strategy and crucial morphological changes of Artemia under indoor mass culture conditions
摘要
Abstract
This study explored a feeding strategy for the indoor mass culture of high-quality and uniform-size Artemia and clarified the crucial stages for morphological changes in Artemia in high-density culture.First,Artemia franciscana cysts from the Great Salt Lake,USA,were hatched and cultured in 100 L conical tanks for 4 days at an initial density of 1 ind./mL.Freshly cultured microalgae Isochrysis galbana was used as the basic diet.Subsequently,Artemia were transferred to 10 L conical tanks at an initial density of 0.5 ind./mL and fed shrimp feed with a size of 25-30 μm.Feeding was conducted at three levels,with total feed amounts of 3.33 g,6.70 g,and 10.02 g,and three replicates were performed.The results showed that with total feeding amounts of 6.70 g and 10.02 g,the body length and biomass of Artemia were significantly higher than those with a total feeding amount of 3.33 g(P<0.05).During the culture process,the overall variation trends of NO2--N,NH4+-N,and total nitrogen(TN)contents were consistent.The NO2--N and NH4+-N contents in each group remained at a low level(0.01-0.04 mg/L and 0-0.12 mg/L,respectively).However,the variation trends of NO3--N differed.With a feeding amount of 3.33 g,the content of NO3--N increased from 0.33 mg/L to 0.53 mg/L;with a feeding amount of 6.70 g,the content of NO3--N increased from 0.33 mg/L to 0.39 mg/L;and with a feeding amount of 10.02 g,the content of NO3--N decreased from 0.33 mg/L to 0.28 mg/L.At the end of culture,the contents of TN in the groups with feeding amounts of 3.33 g,6.70 g,and 10.02 g reached 7.46 mg/L,8.69 mg/L,and 15.95 mg/L,respectively.Subsequently,the feeding amount of 6.70 g per 10 L group was applied to the culture of Artemia in a 1-ton water column for 13 days.Two critical stages,namely,the appendage development period and the second antenna degeneration period,were identified during the culture process,which was characterized by a sudden drop in survival rate.On day 13,the survival rate of Artemia was 72.3％,the average body length was 7.24 mm,and the total biomass reached 2230 g.The crude protein content of the biomass was 52.28％dry weight,the crude fat content was 17.64％dry weight,and the eicosapentaenoic Acid(EPA)content was 7.56 mg/g dry weight,all of which were higher than those of biomass collected from Bohai Bay salt ponds.In conclusion,using microalgae with a small size and easy digestibility in the early stage of Artemia culture,followed by an appropriate amount of formulated feed,can achieve a stable biomass yield.Artemia density changes should be closely monitored at the critical stages during the culture process.The results of this study not only provide strategic guidance for the indoor mass Artemia culture but also offer a methodological reference for obtaining a stable number of healthy Artemia in scientific research when using Artemia as experimental animals.关键词
卤虫养殖/投喂策略/存活率/生物量/水质/营养指标Key words
Artemia culture/feeding strategy/survival rate/biomass/water quality/nutrition analysis分类
农业科技引用本文复制引用
卢钰龙,武心华,闫新荣,万学瑞,董考帅,马浩然,高美荣,隋丽英..室内卤虫高密度养殖投喂策略及卤虫形态变化关键期探究[J].中国水产科学,2025,32(12):1798-1807,10.基金项目
国家重点研发计划项目(2022YFD2400203) （2022YFD2400203）
山东省重点研发计划项目(2023TZXD055). （2023TZXD055）
