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Specific and visual detection of EBOV based on a one-pot RT-RAA-CRISPR/Cas12a assay

Zanheng Huang Pei Huang Zengguo Cao Tianyi Zhang Kaikai Jin Meihui Liu Yujie Bai Zhiyuan Gong Xuemeng Li Yuanyuan Li Haili Zhang Hualei Wang

Virologica Sinica2025，Vol.40Issue(6)：P.1054-1057,4.

Virologica Sinica2025，Vol.40Issue(6)：P.1054-1057,4.DOI:10.1016/j.virs.2025.11.011

Specific and visual detection of EBOV based on a one-pot RT-RAA-CRISPR/Cas12a assay

Zanheng Huang ¹Pei Huang ¹Zengguo Cao ²Tianyi Zhang ¹Kaikai Jin ¹Meihui Liu ¹Yujie Bai ¹Zhiyuan Gong ¹Xuemeng Li ³Yuanyuan Li ¹Haili Zhang ¹Hualei Wang¹

作者信息

1. State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine College of Veterinary Medicine,Jilin University,Changchun 130062,China
2. Key Laboratory of Special Pathogens and Biosafety,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430071,China
3. Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics,School of Basic Medicine,Guangdong Medical University,Dongguan 523000,China The Marine Biomedical Research Institute,Affiliated Hospital of Guangdong Medical University,Guangdong Medical University,Zhanjiang 524023,China
折叠

摘要

关键词

ebola virus disease evd/CRISPR Cas/one pot RT RAA CRISPR Cas assay/Ebola virus disease

分类

医药卫生

引用本文复制引用

Zanheng Huang,Pei Huang,Zengguo Cao,Tianyi Zhang,Kaikai Jin,Meihui Liu,Yujie Bai,Zhiyuan Gong,Xuemeng Li,Yuanyuan Li,Haili Zhang,Hualei Wang..Specific and visual detection of EBOV based on a one-pot RT-RAA-CRISPR/Cas12a assay[J].Virologica Sinica,2025,40(6):P.1054-1057,4.

基金项目

financially supported by the National Key Research and Development Program of China(grant No.2021YFF0703600) （grant No.2021YFF0703600）

the Science and Technology Development Program of Jilin Province(Grant20250601001RC). （Grant20250601001RC）

Virologica Sinica

ISSN：1674-0769

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